FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_C03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_C03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422738
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA58470.4109681473328189No Hit
GAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC55800.3922015156690831No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT50710.3564254275910252No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG50310.3536139471919637No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG39460.2773525413674197No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA36940.2596402148533321No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG35200.24741027511741442No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG34840.24487994275825906No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA34300.24108444421952602No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT30950.2175382958773857No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC30550.21472681547832417No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30320.21311021424886384No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT29320.20608151325121No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG29260.20565979119135078No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA29230.20544893016142116No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA28800.20242658873243No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC28050.19715506298418964No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG27990.19673334092433042No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT27720.1948355916549639No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC25720.18077818965965625No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT24530.17241403547244818No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG24200.17009456414322244No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT23160.16278471510566248No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA23000.16166012294603785No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG21940.1542096998885248No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC21890.15385826483864212No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT21620.1519605155692756No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT21540.15139821948946328No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC21230.1492193221801906No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA20590.14472095354169215No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG20260.14240148221246637No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG19690.13839512264380371No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT19410.13642708636446066No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT19290.1355836422447422No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG18780.13199900473593873No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT18570.13052297752643144No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG18520.13017154247654875No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG18490.12996068144661913No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC18470.12982010742666605No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA18440.12960924639673643No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT18400.12932809835683026No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT18320.12876580227701798No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA18090.12714920104755761No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT17850.12546231280812067No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA17750.1247594427083553No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA17480.12286169343898876No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG17460.12272111941903568No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA17430.12251025838910608No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT17420.12243997137912953No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA17250.1212450922095284No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC17170.12068279612971608No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA17170.12068279612971608No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA17170.12068279612971608No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA17020.119628490980068No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG17000.11948791696011493No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG17000.11948791696011493No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG16960.11920676892020877No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG16860.11850389882044339No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT16820.11822275078053725No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC16800.11808217676058416No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA16760.11780102872067802No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT16710.11744959367079531No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA16610.11674672357102996No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC16590.11660614955107686No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA16490.11590327945131147No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT16420.11541127038147572No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA16410.11534098337149917No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT16360.1149895483216165No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT15760.1107723277230242No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG15590.10957744855342305No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT15460.10866371742372805No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG15310.10760941227407998No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC15240.1071174032042442No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT15150.10648482011445537No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT14980.10528994094485422No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC14820.10416534878522961No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA14790.1039544877553No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC14770.1038139137353469No Hit
CTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCA14500.10191616446598038No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG14490.10184587745600383No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC14350.1008618593163323No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC14230.10001841519661385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG351.0913936E-11145.46451145
CCGTGCG100.0070236893145.4645145
GCGTCCG100.0070236893145.4645145
TTATACG301.08113454E-7121.22041145
AGATCGG256.363818E-6116.371605145
ACAGACG850.0111.237564145
AGTAGAA7200.0110.778631
ATGAACG203.817513E-4109.09837145
CTGGGCG301.5744858E-596.97633145
GTTGGCG301.5744858E-596.97633145
CTATCGT301.598635E-596.679541
TTGCTCG406.0033926E-790.91531145
AGGCTAG406.0033926E-790.91531145
GCATTTA259.269044E-487.2787145
CTCGGCG259.269044E-487.2787145
GAACCCG259.269044E-487.2787145
GGAGGCG259.269044E-487.2787145
CCGATCC259.406227E-486.956534
GCAGGTA3050.080.813382
TGGACCG950.076.560265145
Produced by FastQC (version 0.11.5)