FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_C02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_C02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072040
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA14190.13236446401253685No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA12790.1193052498041118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG203.81763E-4109.095665145
CAATTCG2900.057.478269
AGCGAAA2150.053.970861
GGTCGTT1751.8189894E-1245.5542037
GCGAAAG2650.043.7876741
CGAAAGC2900.039.9960752
TACTACG800.002040463336.2362984
GTAGAAA9450.035.3023641
CAAGACG2900.032.603302145
CCTTATA9000.032.219062
ATGAACG2755.456968E-1231.622936
TAACGTT950.004736196230.5147745
TCGCGAT200.006073883729.002838110-114
TACCGTA1255.1570445E-428.9971542
TATACTG13500.028.9890375
AGTAGAA10550.028.8718531
TTATACT12150.028.0346664
ATACCGG1050.007696594627.627941
ATGCGTC1855.8337664E-627.4220647
TCGTTTT2705.5861165E-926.8417029
Produced by FastQC (version 0.11.5)