FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459767
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA38120.26113756510456804No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT36750.25175250570810276No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC35480.24305248714349617No Hit
ATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAACATCG30930.21188312929392156No Hit
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT27700.18975631042488286No Hit
GTTGAATATATTGTGGACCTCTAGGTCGACAGCCCTGAAGCTATTTTAAA27250.18667362668151835No Hit
GTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATTTTTAG24220.16591688947619723No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC22990.15749088724433422No Hit
CCATAGTGGTGTATGGTGACAGACGGTGGCTCCTTTTTTCCTGAGCACAG21850.14968142176114407No Hit
TAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAAC21850.14968142176114407No Hit
ATATTCAACTGTCTCTCCTTTGACCGATGTTCGAGTTATGTTTTGTTTTC21370.14639322576822192No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC21090.14447511143901734No Hit
ACCCAGGACCTATTATTTAGTGGGAAAAATGAAGTGCAAAATAAACGTAT20940.14344755019122915No Hit
ATGTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAAC20620.14125541952928106No Hit
ATTTTAAAAGGTGCATTAGATTTATCCCTACCCAGGACCTATTATTTAGT20150.13803572761954477No Hit
GGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAAC19820.13577509287441078No Hit
CTTATAGACTTGTCTAATCACTGTATTATACATCTGAAACTAATGTAACA19270.1320073682991875No Hit
CTATTATTTAGTGGGAAAAATGAAGTGCAAAATAAACGTATGATCCCACT18990.1300892539699829No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT18770.12858216413989357No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC18600.12741759472573363No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG18350.12570499264608667No Hit
CCCTACACCTGCTTCAACAGGAGCGTGAAGAAAAGACACACACCAAACTG18070.12378687831688208No Hit
GAAGTGGGATCATACGTTTATTTTGCACTTCATTTTTCCCACTAAATAAT17970.12310183748502329No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA17800.12193726807086336No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA17670.12104671498944694No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC17290.11844355982838356No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA16980.11631993324962135No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC16830.11529237200183318No Hit
CCAGTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATTTT16740.11467583525316026No Hit
CTAAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATA16720.1145388270867885No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT16680.11426481075404499No Hit
AAGTAATATTTTCCCCCTTCTTAAATAAAAAGCATAAATCCTTAGGAATG16430.11255220867439804No Hit
ATTATTTAGTGGGAAAAATGAAGTGCAAAATAAACGTATGATCCCACTTC15780.10809944326731595No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG15770.10803093918413008No Hit
GTTATAATCTCTCCTTTCCCTCACTCCCCTTTTCCCTCCTTCTGAACTCA15740.10782542693457244No Hit
GTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGCTTCAGGGCT15620.1070033779363419No Hit
ATGTATTAGTTTAGGGAAAATTAATGAAGATAAAAACAATAACAAGCACA15470.10597581668855371No Hit
GTGTTATACGCTACCCACTTGTCTTTACATCGGATAATTAACTCTATTAA15350.10515376769032318No Hit
CAATATATGTATATTATCATTAATATACAATATTATTTATGAAAATGTAA15350.10515376769032318No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT15250.1044687268584644No Hit
GTATAGTCAATGGTATTACCATAGTGGTGTATGGTGACAGACGGTGGCTC14840.10166005944784338No Hit
GTATAATACAGTGATTAGACAAGTCTATAAGTTATGCTGTGCTCAGGAAA14670.10049549003368345No Hit
GTATTACCATAGTGGTGTATGGTGACAGACGGTGGCTCCTTTTTTCCTGA14670.10049549003368345No Hit
CACCAGTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATT14640.10028997778412582No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG14610.10008446553456819No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCG256.3631196E-6116.37438145
AGCGAAA2550.099.5536651
TCGCCGG300.001911331872.733986145
GCGAAAG3500.072.5070952
CGAAAGC4100.061.8750953
ATGAACG5150.052.0639236
ACGGGCG701.6643307E-551.952847145
GTAGAAA8600.049.76031
GGACAGT11000.042.8216176
CACATCG1703.8198777E-1142.784695145
TAGATCG1053.7415284E-641.56228145
AGTAGAA8800.041.211341
GCTACCG907.343328E-540.40777145
GCCGTAG750.001460301538.79146145
CGACCAA3600.038.246665
ACCGTGC2750.036.896268
ATACACG800.002004956636.366993145
GTCTAGT2252.0008883E-1135.4600681
TAGAAAC12500.034.8034062
CCATCGG6900.034.6618589
Produced by FastQC (version 0.11.5)