FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_B12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_B12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030078
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT39400.382495306180697No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA30460.29570576208791954No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC29610.2874539597972192No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA23340.22658478289993575No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG22910.22241034174111085No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT19220.18658781179677655No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA18570.18027761004506454No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG17170.16668640627214637No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC16960.16464772570620867No Hit
ACTATCCACAGAGTAAAAATTCAATCCACAGAATGGAGAAAAGATTTGCA16270.15794920384669897No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG16230.15756088373890131No Hit
GTTGTAAACTGCACGGGAGTCGGGATCCTGACATGGAGACCTCTCCGTAC16030.15561928319991303No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG15510.1505711217985434No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA15290.14843536120565626No Hit
GTCTATGACCCAGCACTCCTGCTCCCAGATATTCTCCCAAGAGAAGCAAA13540.13144635648950856No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT13210.12824271560017786No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA12750.12377703436050475No Hit
TGGATAGACAGACTGGATTCATGAAAATTAAGAGATTTTATGCTTCAAAA11920.11571939212370326No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA11850.11503983193505735No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT11690.1134865515038667No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG11100.10775882991385119No Hit
GTATTATCTCACATGCAAAAAATATCTCAGTAAGAAAAAATGCAACCCAC10990.10669094961740762No Hit
GTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATA10810.10494350913231812No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC10790.1047493490784193No Hit
ATGTAGGACTGTCCCCTCCCCAAGCAGAATGAGAGACCTGTGCTGACAGA10560.10251650845858275No Hit
CCCTAATGGTTAGTGATATTGGGCATCTTTTCATGTGTTTATAGGCCATT10350.10047782789264503No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGC100.007029702145.42093145
CATATCG100.0071026455144.919714
CGTATCG256.3724583E-6116.336754145
TGACGCG608.112693E-1084.82889145
ATACACG606.6897446E-660.59206145
AGCGAAA5500.055.37331
CGCCGTT400.00598193854.53285145
TATTATC4350.053.334872
CAATTCG701.7001228E-551.764589
ATACTAA3900.050.171827
CATACTA4400.046.1130566
GTATTAT6650.045.7974661
CCGTATA657.2326633E-444.744904145
CGAAAGC6950.043.7993163
CCGCTTC2700.042.9454279
CGACGAG855.2510135E-542.770866145
GTAGGAC4450.040.7176673
TATATCC2850.040.689093
TATCGGG907.3558214E-540.394703145
CCATACG907.496332E-540.2652473
Produced by FastQC (version 0.11.5)