FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_B11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_B11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806702
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG9370.11615193714655472No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA9260.11478836050982891No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG8520.10561520859003695No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT8180.10140051716742986No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG203.8209715E-4109.06918145
TGACGCG406.56636E-572.71279145
CAATTCG3650.045.67139
GGTCGCG700.001041393141.550163145
AGCGAAA1859.640644E-1139.1920741
TAGAAAC8800.038.7076342
CAGCTCG4100.031.8197679
GTAGAAA11700.031.6048891
GCTATAC3000.031.3975583
CCGACGG950.004659475330.61591145
CGAAAGC2401.5916157E-930.189963
GTATAAT2202.2613676E-829.6612721
TATATAC9750.028.2392233
TATGCGT2603.7343852E-927.8728356
ATGCGTC2903.965397E-1027.4917937
GTATTAT3700.027.4344521
TATACCA4250.027.2808975
GCGAAAG2705.5806595E-926.8421732
AAGGTCG5200.026.482489
GTTTCGT2507.849485E-826.0922099
Produced by FastQC (version 0.11.5)