FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_B10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_B10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421604
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG12990.30810903122361266No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG12000.2846272805760856No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT11770.279171924365044No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT10850.2573504995208774No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT8770.2080151042210226No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC8360.19829033880133964No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC8030.1904630885854973No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA6710.15915408772212786No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT6520.15464748911300652No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT6340.15037807990436525No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA6130.14539710249428373No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA6120.14515991309380366No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA6080.14421115549188337No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC5840.13851860988036166No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT5810.13780704167892147No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT5550.1316401172664396No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG5510.1306913596645193No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA5390.12784508685875848No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA5300.1257103822544378No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA5210.12357567765011718No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG5190.12310129884915703No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT5190.12310129884915703No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT5120.12144097304579653No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC4960.11764594263811538No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG4870.11551123803379473No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG4870.11551123803379473No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT4860.11527404863331467No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT4670.11076745002419333No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG4650.11029307122323317No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT4610.10934431362131289No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT4600.10910712422083281No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG4520.10720960901699225No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG4460.10578647261411184No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC4400.10436333621123138No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG4380.10388895741027125No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT4380.10388895741027125No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG4370.10365176800979119No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA4350.10317738920883103No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT4290.1017542528059506No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC4260.10104268460451041No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG100.0070227217145.45909145
GGTCGCG100.0070227217145.45909145
CGACGAG100.0070227217145.45909145
GTTGTCG100.0070227217145.45909145
GGAACCG100.0070227217145.45909145
CCGACGG100.0070227217145.45909145
CGCCGTG100.0070227217145.45909145
AGCGAAA553.6379788E-12105.4619751
GCGAAAG607.2759576E-1296.639062
GGTACTG1450.094.950345
CAGGTAC1500.091.8071063
GCAGGTA1550.088.845592
GGGGGGA451.2086984E-680.810616145
GTAGAAA5850.069.406591
AGTAGAA4300.069.1327741
AGGTACT2200.062.5809064
GTCATAG350.003564077262.1472321
GTTAGTA350.003569108262.125112
CGAAAGC1007.203198E-1057.9834333
CTGACGG651.0722784E-555.945812145
Produced by FastQC (version 0.11.5)