FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_B09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_B09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580058
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT9590.16532829475673122No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC9040.15584648431708553No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT7500.12929741508607762No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC6580.11343693216885208No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC5810.10016239755334812No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA900.080.578651
GGTCGCG300.001914670972.69638145
GCGAAAG1301.8189894E-1255.770792
CGAAAGC1353.6379788E-1253.7005773
GCATTAA3950.047.7352031
CATTAAG4300.047.2106252
ATTAAGA6250.032.4781073
AGATGTA7250.031.989867
GTAGAAA4800.031.7278441
TATAAGG1601.8749088E-631.7196372
TAAGATG7100.030.6240945
TACGCCC1204.061452E-430.2013634
TTAACGT3500.028.9933094
ATGAACG1000.00608179228.990816
TAACGTG3550.028.5824875
TACCTAT1855.8249498E-627.4237387
CTCTACG1606.8099616E-527.1952951
CGTGTTC3503.6379788E-1226.9200368
TTAAGAT7350.026.6265074
TTGCACG2207.302333E-726.435051145
Produced by FastQC (version 0.11.5)