FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_B04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_B04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851168
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC12400.1456821685025753No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC9240.10855671265837061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2650.076.606171
GCGAAAG4600.045.6945152
CGAAAGC4350.044.982893
CTAGTAC2900.037.485743
GTAGAAA8250.036.910241
TAGTACT3300.032.9420174
GACAGTG15100.031.194917
GGACAGT14300.030.4080146
TAGAAAC10600.028.7188682
GAACGTC2456.463415E-826.6209628
TAAGACC5500.026.3536134
CGTATGC1100.00969150826.3520648
TAAACCG1450.001229014124.9904945
GCAGGAC15150.024.3966643
GTCTAGA2751.9673098E-723.7280141
CAGTGGG18200.022.6960759
ACAGTGG21700.022.3749668
CAAGGGT5250.022.0868364
CTATTAC2303.140552E-522.0659071
ATCGCTG6800.021.39086145
Produced by FastQC (version 0.11.5)