FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_B03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_B03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920372
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT45290.49208363574728475No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT25240.2742369389768485No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC17080.18557713620144897No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA16230.17634174007901152No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT13210.14352892091458672No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA12680.13777037980294923No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA12140.13190318697222428No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT11020.11973419443442435No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA10750.11680059801906185No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG10160.11039014659289939No Hit
GACTAACACAAACACACAATTACAAAGTGGTAAGTGGTTTATTTTCAAAT9930.10789115705388691No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA9520.10343643657129943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2000.083.3809361
GCGAAAG2900.057.4947172
TCCGTCG400.00597519654.547928145
ATCGCTA553.1687942E-452.894962145
GACAGTG17500.050.5217327
CGAAAGC3500.049.6989143
CGGTTCG604.943137E-448.3367771
GTAGAAA9200.048.0740781
GGACAGT18500.047.7908336
CAAGGAC18050.044.9722373
CGACCAA1105.2809464E-639.526785
GTATACG2300.037.814393
AGGACAG24350.037.2000855
TATACGT2950.036.8490034
CAGTGGG23300.036.3903669
ACAAGGA24900.034.0631372
GTATACC2603.6379788E-1233.4475564
AAGGACA25000.033.3360634
CGAAGTA1102.4427622E-432.9389845
AACAAGG28550.031.490861
Produced by FastQC (version 0.11.5)