FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621366
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT11110.17879961246672652No Hit
CAATATACACAGTGCTTAGTACAAGTACTTGTTGGTAGTAACTGCTGGTG6520.10493010560603575No Hit
TCTATAGACAGTGCACATTCAGATGTTGCTTCGGGAATGAGTGATGGCAA6420.1033207481580904No Hit
GTACATAGCTAGGAAATAACAAAACTGAGTCTTCAGCCTGAATTACTGCA6410.10315981241329586No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG100.0070220428145.46933145
TTATACG100.0070220428145.46933145
AGCGAAA2850.0101.730321
GCGAAAG3200.090.5889742
TCAATCG259.265336E-487.2816145
TGTACGT259.399897E-486.9654162
CGAAAGC3850.077.164683
GCTTTCG350.003520502762.344145
TTCGCCC1051.0768417E-955.41689145
ATCGTGC858.9537207E-751.1478928
GGGTTCG1004.7200956E-850.721669
TATGCGT450.00966486648.2985656
TAGGTTG951.9292365E-645.756545
GTACAGG803.7382284E-545.301781
ATCAACT1307.385097E-944.586884
TCGTGCG1105.281303E-639.523379
TTGTGCG1209.594694E-636.2297559
ATATATG3500.035.2059561
TATAGGA1707.842027E-834.1040842
CCTAATA1102.4407826E-432.9414442
Produced by FastQC (version 0.11.5)