FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_B01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_B01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394902
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT20040.14366600664419435No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG17890.12825273746829527No Hit
ATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAACATCG16540.11857463821831211No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA14310.10258785204982142No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG606.6749944E-660.61652145
CCGACGG501.977966E-458.191864145
AGCGAAA2800.054.3650631
GCGAAAG2900.052.480992
CGAAAGC3050.049.8874363
GTAGAAA8700.041.659051
TGTATCG700.001039751841.565613145
ATGAACG2900.037.478366
ATACACG1051.8184517E-434.638012145
CCATCGG3850.031.995669
ACCATCG4100.031.8109028
ATACCTA6900.031.5035486
AGGACCG1453.4135417E-530.099237145
TACCTAT6500.030.0980037
GGACAGT14100.028.263826
TTAAGCG1555.5236404E-528.048327
TAACGAC1907.192257E-626.6941574
TTAGCCG1100.00951840126.450844145
CAATTCG1100.0096984326.3493659
TAGAAAC14500.024.990952
Produced by FastQC (version 0.11.5)