FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_A12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_A12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365141
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTGTACCATTATCAATTAATTTGGCCCATTCACAAACATAACTCTTA33310.9122503361715063No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT30570.8372108308845075No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA27260.7465609175633522No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC20750.5682736258048261No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT17750.486113583519791No Hit
ATTTTTAACAGGATCAGCACTGGAGGAACGCAACAATGCATTTTATTAAG17330.4746111775998861No Hit
AATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAA16600.45461890064386085No Hit
CCTTCCAGATCATGATATAGAATACTTAAGTCAACAAAGAGCATTACCTC16410.44941543129914197No Hit
CATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGC15860.43435275688021885No Hit
ACCATACACAGGCAGATGAGTATTTACATGTGTAATCACCTTCCAGATCA15470.42367195138316427No Hit
GTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAG15360.42065941649937966No Hit
GTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGTCCTAATTA15200.4162775475775112No Hit
GTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGG15020.41134794504040906No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA14350.3929988689300845No Hit
GTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGAT14140.3872476659701321No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT13970.38259193024064675No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC13520.37026792389789154No Hit
ATATAGAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAG13430.36780312262934045No Hit
GTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAATTAATT13320.36479058774555584No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG12500.3423335095209796No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG12490.34205964271336275No Hit
GTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGCACTGGAG11700.3204241649116369No Hit
TATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGCAC11650.31905483087355296No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC11550.3163161627973851No Hit
GGTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCA11460.3138513615288341No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT11170.30590922410794735No Hit
GGAATGTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTT10960.3001580211479949No Hit
CAACAATGCATTTTATTAAGGAAATTCCAGTCTGTGGTTAGGTATAATTG10700.2930374841499585No Hit
TAATTGTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGTCCT10050.2752361416548676No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA9920.2716758731558494No Hit
TTATTAAGGAAATTCCAGTCTGTGGTTAGGTATAATTGGGATTAATTGTT9810.2686633382720648No Hit
TTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATC9790.26811560465683115No Hit
GTGGTTAGGTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAA9750.26702013742636405No Hit
ATAGAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTC9610.2631860021197291No Hit
TCATGATATAGAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGT9430.25825639958262697No Hit
GCATTTTATTAAGGAAATTCCAGTCTGTGGTTAGGTATAATTGGGATTAA9300.25469613108360883No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC9160.2508619957769738No Hit
ATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAATTAATTTGGCC9040.24757559408557242No Hit
AGTAGAAACAAGGAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACA8810.2412766575103864No Hit
GTGCTGATTTGTACCATTATCAATTAATTTGGCCCATTCACAAACATAAC8620.23607318816566752No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA8340.22840491755239758No Hit
ACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAG8280.22676171670669687No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG8280.22676171670669687No Hit
GAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATAGAACAATTAATCCCA8240.2256662494762297No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG8220.22511851586099618No Hit
GCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGC8110.22210598097721154No Hit
GAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTA8080.22128438055436117No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG8050.22046278013151083No Hit
GCACTGGAGGAACGCAACAATGCATTTTATTAAGGAAATTCCAGTCTGTG7950.217724112055343No Hit
CTCTTAATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATA7860.21525931078679195No Hit
TTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGG7810.21388997674870805No Hit
ATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGTCA7680.21032970824968983No Hit
ATTCCAGTCTGTGGTTAGGTATAATTGGGATTAATTGTTCTATGAAGACA7480.20485237209735419No Hit
GATCAGCACTGGAGGAACGCAACAATGCATTTTATTAAGGAAATTCCAGT7420.20320917125165347No Hit
CTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACC7390.20238757082880313No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA7360.20156597040595275No Hit
ACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAAT7170.19636250106123387No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT7080.19389769979268281No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT7070.19362383298506605No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA6940.19006356448604786No Hit
GATGAGTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACT6910.1892419640631975No Hit
AACCAACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTA6890.18869423044796393No Hit
AATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATAGAACA6890.18869423044796393No Hit
GTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGTCAACAAAGAG6860.1878726300251136No Hit
ATGATATAGAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTT6700.18349076110324505No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC6640.18184756025754434No Hit
TCCTAACCATACACAGGCAGATGAGTATTTACATGTGTAATCACCTTCCA6620.18129982664231079No Hit
GATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGT6470.17719182452805904No Hit
GTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCACCCGCATAAT6370.1744531564518912No Hit
GTCAATAACCAACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTA6320.17308382241380726No Hit
GAAACAAGGAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACATTTC6280.17198835518334013No Hit
GCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGCACTGGAGGAACG6240.170892887952873No Hit
TGCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATG6200.1697974207224059No Hit
GAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAAATCAGC6180.16924968710717228No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA6080.16651101903100446No Hit
GTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAATGTGCAATA5930.1624030169167527No Hit
GTAGAAACAAGGAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACAT5900.16158141649390234No Hit
GGCAAATGCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGA5860.16048594926343523No Hit
TCTCTGTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGAT5840.15993821564820165No Hit
ATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTA5650.15473474630348277No Hit
ATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTTCCTGGTAACTCG5630.1541870126882492No Hit
GTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTT5540.15172221141969813No Hit
ATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAATAA5480.15007901057399745No Hit
ATGCAAATCCTAACCATACACAGGCAGATGAGTATTTACATGTGTAATCA5440.1489835433435303No Hit
GTGTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAG5370.14706647569021283No Hit
GTACAAATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGA5370.14706647569021283No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT5260.1440539408064282No Hit
GCATTACCTCATCCCCAGTTTCAGTCAATAACCAACCCTGTCCTATCCTT5250.1437800739988114No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG5170.14158913953787713No Hit
GAATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAAT5140.1407675391150268No Hit
TACACAGGCAGATGAGTATTTACATGTGTAATCACCTTCCAGATCATGAT5130.14049367230741003No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG5090.1393982050769429No Hit
ACATAACTCTTAATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTC4980.13638567019315825No Hit
GTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTAT4960.1358379365779247No Hit
CAATTAATTTGGCCCATTCACAAACATAACTCTTAATTAGGACAGAGAAA4900.13419473573222399No Hit
GTAATCACCTTCCAGATCATGATATAGAATACTTAAGTCAACAAAGAGCA4850.1328254016941401No Hit
CCTAAATTCCGTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATAC4750.13008673361797224No Hit
CAATTATACCTAACCACAGACTGGAATTTCCTTAATAAAATGCATTGTTG4700.12871739957988831No Hit
AATCCTAACCATACACAGGCAGATGAGTATTTACATGTGTAATCACCTTC4690.12844353277227152No Hit
GGCAGATGAGTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAA4680.12816966596465476No Hit
CTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGCCAGAGAGT4670.12789579915703797No Hit
GGAAATTCCAGTCTGTGGTTAGGTATAATTGGGATTAATTGTTCTATGAA4660.12762193234942118No Hit
ACCCAATTTTCTCTGTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAA4600.12597873150372046No Hit
AAACAAGGAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACATTTCA4570.12515713108087012No Hit
ACATATTCCCTATTCTTTCTCTCTGAAACTTCTGTCAGTTGGCTGTTTTA4560.12488326427325334No Hit
TTTCAAGTCCTATTTTTAACAGGATCAGCACTGGAGGAACGCAACAATGC4560.12488326427325334No Hit
GTTAGGTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAA4540.12433553065801979No Hit
AATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGACTGAA4530.124061663850403No Hit
GGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTG4480.12269232981231909No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT4450.12187072938946873No Hit
GAACGCAACAATGCATTTTATTAAGGAAATTCCAGTCTGTGGTTAGGTAT4450.12187072938946873No Hit
GTCCTATTTTTAACAGGATCAGCACTGGAGGAACGCAACAATGCATTTTA4420.12104912896661837No Hit
ACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAATAACCAACCCTGTCC4400.12050139535138482No Hit
GGGTTACATAGAACTGATTTTTAGCATGTGCTCCAACTGAGCTTTGAGGA4360.11940592812091767No Hit
AATTAATTGATAATGGTACAAATCAGCACAGAGTTTTCCAAGGATAGGAC4330.11858432769806732No Hit
ATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGCA4280.1172149936599834No Hit
ACCTGAGCCAAAGGCAAATGCTTAATGATCCTCTATTGGTGCTTCTTACA4160.113928591968582No Hit
ATAACCAACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCAT4070.11146379070003094No Hit
ATCACGACCTGAGCCAAAGGCAAATGCTTAATGATCCTCTATTGGTGCTT4050.11091605708479738No Hit
GTACCATTATCAATTAATTTGGCCCATTCACAAACATAACTCTTAATTAG4020.11009445666194703No Hit
GATATAGAGTGGTAATAAGGGAGGAAGGTATGAATATAGTTAGTGACCCA3990.10927285623909667No Hit
CTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGT3970.10872512262386311No Hit
TCATAGAACAATTAATCCCAATTATACCTAACCACAGACTGGAATTTCCT3970.10872512262386311No Hit
GGATAGGACAGGGTTGGTTATTGACTGAAACTGGGGATGAGGTAATGCTC3960.10845125581624632No Hit
TTTGTACCATTATCAATTAATTTGGCCCATTCACAAACATAACTCTTAAT3930.10762965539339597No Hit
GTTCCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAA3930.10762965539339597No Hit
CTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGTCCTAATTAAGA3910.1070819217781624No Hit
TGTAATCACCTTCCAGATCATGATATAGAATACTTAAGTCAACAAAGAGC3910.1070819217781624No Hit
GTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTTTGGTTG3870.10598645454769527No Hit
GAATGGAACATTTACCATATGTATGAGTTACTAATTTCCTGGACATTATC3870.10598645454769527No Hit
AATTTGGCCCATTCACAAACATAACTCTTAATTAGGACAGAGAAAATTGG3840.10516485412484491No Hit
ACTCTGTGCTGATTTGTACCATTATCAATTAATTTGGCCCATTCACAAAC3780.10352165327914423No Hit
GAAAATTGGGTTGTTTGTCAGGTTGTCTTCATAGAACAATTAATCCCAAT3770.10324778647152744No Hit
GGTAAGTCTTGATTGCCAATATTGCACATTTCAAACATGAATCCGTAGAC3700.10133071881820994No Hit
TTATCAGCCATTAGCTGGTTTCCTGTGTCCCAGCAAATGCTGAGGTAAGT3700.10133071881820994No Hit
ACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAATAACC3670.10050911839535959No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCG151.2149841E-4145.429145
CGACGAG100.007026684145.429145
GCCGACC253.6417987E-8145.429145
TAGATCG253.6417987E-8145.429145
GACCGTG100.0070962245144.95088
TCGCCGG256.3651296E-6116.34319145
CCTGTCG203.8189132E-4109.071754145
CGCCGTG203.8189132E-4109.071754145
AGTAGAA5200.089.2738951
TGCCCCG259.2724524E-487.2574145
TGACGCG259.2724524E-487.2574145
CCAACCG608.076313E-1084.83359145
TTGTTCG353.384375E-583.10229145
AGCGAAA502.2966979E-672.535031
AGTGGTA1300.072.475418
TAAGATG3400.066.080525
CCAATTG350.00352320462.326717145
GGGACTT350.003569483362.121785
GCGAAAG606.7995898E-660.4127272
ATAGAGT1800.060.38794
Produced by FastQC (version 0.11.5)