FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_A10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_A10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701017
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG7460.1064168201341765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG203.8187474E-4109.08362145
AGCGAAA1700.068.219881
CGAAAGC2350.049.3364643
GCGAAAG2500.046.3795852
CGCTGCG1354.850699E-737.707916145
TATATAC6900.034.656183
CAGCTCG3800.032.4199379
TATACTG5100.031.2585165
CCTTATA4600.029.9324762
TCGCCGG1000.005983009529.088963145
TATGGAC1255.1677314E-428.9851683
TTATAAC1555.5173023E-528.0501653
GACCGTA1050.007723471627.6068956
GACTAAC1358.0909545E-426.8457851
CAATTCG3709.094947E-1225.4617639
TATAACA2301.1065622E-625.2062952
GTTATAT1450.001227692824.994351
TTATACC2051.2946686E-524.7434394
GTATAGA3301.7753337E-924.1612071
GTATATA6950.023.9822482
Produced by FastQC (version 0.11.5)