FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_A09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_A09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916850
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT15710.17134754867208377No Hit
GTGCAAACCCTGGTTTACATGTGCGGCGATATGCTCAGCAACGTCGGGGC13760.1500790750940721No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT12340.13459126356546872No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC12280.1339368489938376No Hit
GGTCAGGGCGGTTGGGATACGCAAAAGAGACGTCTTGGAATTCCACCAGC11970.13055570704041009No Hit
ATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAACATCG10840.11823089927469052No Hit
GTACAAATTCTGCAGCAAGGCAGCCACTGTGCTCTTCCCGGACCCATTGG10220.11146861536783552No Hit
GTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATTTTTAG9820.10710585155696134No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC9760.1064514369853302No Hit
TCTGTACTCAGTCCGTGCTCCAACTCCTGTATGAAGCCATGGGCATTAGC9450.10307029503190271No Hit
TAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAAC9300.10143425860282489No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG151.2139365E-4145.47842145
GGTCGCG259.2644576E-487.287056145
CTACTCG502.2613876E-672.73921145
CCCGAAC350.003569296662.1301573
CGGTTCG3050.054.6967741
CGAAAGC1652.7284841E-1143.94482
GGTTCGC4100.042.444252
GCATTAA5300.042.4247741
GTAGAAA5350.042.028281
TTCGCAG4200.041.415594
GTTCGCA4300.040.4568443
TGGTGCG3850.037.6464277
TCGCAGT4700.037.0076525
CAATTCG800.002040208836.236669
CATTAAG6500.035.69672
AGCGAAA1854.1636667E-935.286171
GCGAAAG2154.838512E-1033.736131
CGCAGTT6400.032.8376856
GGTGCGG4300.032.0230948
CGAAACC1851.6494778E-731.3381066
Produced by FastQC (version 0.11.5)