FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_A07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_A07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970149
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG13740.14162772934879075No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA11600.1195692620411916No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT10560.10884925923749858No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT10230.10544771988632674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATACG259.2809624E-487.24835145
TATACCG300.001913957172.706955145
TGACGCG451.1771168E-464.6284145
CGTGCTA2300.047.2684568
AGCGAAA1751.8189894E-1245.609521
TATACGT2250.045.0952764
CGAAAGC1801.8189894E-1244.299143
CAATTCG3000.043.4869779
CCTTATA5300.039.6906782
GCGAAAG2057.2759576E-1238.92292
GTATTAC7350.035.5398831
GTAGAAA7200.034.2647251
ACATCAA6900.031.5090526
TGTATAC3500.031.0877722
TTATACT8200.030.9342654
TAGACAG6400.029.4397545
TCCGTGG1000.00608326128.9913188
TATTACA7800.028.8292582
CTAGTAC1306.488294E-427.880583
ATAGACA7300.027.8041953
Produced by FastQC (version 0.11.5)