FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045242
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA32050.3066275561066241No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC18330.17536608747065272No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG16190.15489235985542105No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG14010.13403594574270838No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG12910.12351206706198183No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG12800.12245967919390917No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT11370.10877863690896464No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC10640.10179460832993699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG400.00597165754.556618145
AGCGAAA4150.043.65281
ATGAACG5850.042.1216776
GAACGTC5750.040.331468
GCGAAAG4800.037.7378732
CGAAAGC5200.034.834963
TGAACGT7000.034.1647037
TAAGACC4650.032.7223854
AACGTCA7350.031.5518239
GTCCGTA950.00473900130.5110111
GTAGAAA9850.029.4268651
GCTTATG8950.028.335032
ACCATCG6800.026.6435998
GGCTTAT14900.026.2619931
AGTGATC19250.025.976478
CTAGTAC1751.2608807E-424.8423023
AAGTGAT21050.023.7552037
GTATAGT2751.978351E-723.7153781
GTGATCA20200.023.6785099
CTATACT5550.023.4994754
Produced by FastQC (version 0.11.5)