FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_A02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_A02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459741
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT73730.505089601511501No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT44540.30512262106771No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA29730.2036662668240462No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT25570.1751680606354141No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC25230.17283888032192013No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG23830.16324813785459202No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA22750.15584956509408177No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA20680.14166896730310377No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG19920.13646256424941136No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT19660.13468142636262187No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG19530.1337908574192271No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA17290.11844566947150213No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA17020.11659602628137458No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT16800.1150889096079373No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC16520.11317076111447166No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA16240.11125261262100605No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC15920.1090604429141882No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT15160.1038540398604958No Hit
GACTAACACAAACACACAATTACAAAGTGGTAAGTGGTTTATTTTCAAAT14910.10214140727704435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3350.077.927471
CGTATCG406.560326E-572.73027145
CGAAAGC4750.053.4107483
GCGAAAG4900.053.264162
CGGGTCG450.00951999748.486847145
GGACAGT29400.045.3591846
GACAGTG31000.043.7179457
AATCGCG855.243667E-542.782513145
CAAGGAC30950.040.5172233
ACAAGGA32400.039.6056332
TATTGCG750.001460667638.78948145
CAGTGGG32550.038.5189939
GTAGAAA13450.035.5839841
AGGACAG41350.033.8280145
ACAGTGG41650.032.7131738
AACAAGG42550.031.1877771
AAGGACA42350.030.2921054
CGAAGTA2252.8279828E-828.9904335
AGTAGAA13650.028.1563341
TAGTACT5600.027.1794624
Produced by FastQC (version 0.11.5)