FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399254
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA89300.6381972107994688No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC44130.31538233944659083No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG40320.2881535446745194No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG37380.26714234870866904No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG36100.2579946171317002No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG33190.23719782112468502No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT31660.2262634232240894No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC30550.2183306247471867No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT28660.2048234273405686No Hit
GTTATAAACATATGCATACAAGGTTGTGTGAAATTATGTTTATTTCTCTA25500.1822399650099267No Hit
GTGTATGAGCTGCAGGCCAGTCGTGTGTCCAGTGATGTTATTGATCAGAA21050.1504373044493709No Hit
GAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAATGGCTTCAGCC20800.14865063812574414No Hit
ACTCTGGATCATGGAGATGACTACTGGAAGGGTCCCGTGTCCCCGGGCTC20280.14493437217260055No Hit
GTTGAAGAGATTGCAGTAGTTGTAGTAGGACTCAAACCTCTGCTCCAAGG19580.1399317064664457No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC19290.1378591735310387No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC19290.1378591735310387No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG18810.13442877418967533No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG17750.126853308977498No Hit
AACAAGGGGAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGC17740.12678184232455292No Hit
ACTATAGACTTTGTATCAAGAATAGATAACGAACTCTTAAAACCCAACAA17650.12613864244804732No Hit
GCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTGTAGTAGGACTCAAA17300.1236373095949699No Hit
GTATTTCCACAAAACCGTCTCAGATTTGATTGACCAGAAGGTGTATGAGC17060.12192210992428823No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC16950.12113597674189246No Hit
ATACAAGGTTGTGTGAAATTATGTTTATTTCTCTACGGCAGAAAACAAGA16880.12063571017127699No Hit
TCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTGGAGCAAT16520.11806291066525447No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG15690.11213117847081373No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT15510.11084477871780249No Hit
GACTATAGACTTTGTATCAAGAATAGATAACGAACTCTTAAAACCCAACA15410.11013011218835178No Hit
CTCTACGCCCTGGTCAGATGACGGCTCTGGTGGGGCCCAATGGGTCCGGG15150.10827197921178No Hit
ATTATGTGCAGACCCTGGTTTACATGTGCGGCGATATGCTCAGCAACGTC15090.10784317929410957No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC15080.1077717126411645No Hit
CTATAAGGAGACCCTGGAACGGTGCCGGCAGCTGTGGTGGCGACGAGACC14880.10634237958226313No Hit
ATGTATACGTGCTATTTACATAATAAGGAAAATGTACTTTAAATAGATGT14780.10562771305281243No Hit
GATGTATTATGCCCCAATACTCAGCAGCAGGGAAATGAGGATCTTGGAAA14600.10434131329980119No Hit
AGTTGTAGTAGGACTCAAACCTCTGCTCCAAGGAAGGCCCTCCACTGACT14600.10434131329980119No Hit
GGATCTGGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCC14380.10276904693500966No Hit
GTAGAAACAAGGGGAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAG14140.10105384726432798No Hit
TCTGTACTCAGTCCGTGCTCCAACTCCTGTATGAAGCCATGGGCATTAGC14110.10083944730549278No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA14050.10041064738782236No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCG755.4569682E-1177.56299145
TGACGCG607.870585E-872.7153145
AGCGAAA3750.069.5994641
CGAAAGC4450.058.636563
CCGACGG400.00598081254.536476145
GTAGAAA19050.053.280431
GCGAAAG4900.053.2515642
CTATCGA553.2235493E-452.713673
CGCGTTA450.00952765748.47687145
ATGAACG11550.048.3139576
TAAGATG12750.047.175475
GCTTATG12750.045.4784622
GCGATCT803.741908E-545.299199
ACCATCG14600.044.6786548
CCATCGG15100.044.1592149
GGGCGAT1002.7418082E-643.4841167
GCTACCG855.2499257E-542.77371145
TGTTAGA2250.041.8780862
CATTCCG700.001041479141.5516145
GACCATC15900.041.022757
Produced by FastQC (version 0.11.5)