FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727105
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG48530.28099044354570224No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT41300.23912848379224194No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG38630.2236690878666902No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC38200.22117937241800584No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT37900.21944236163985398No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT37740.2185159558915063No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA37130.2149840339759308No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG34700.2009142466729006No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA34590.2002773427209116No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT33900.19628221793116227No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA33680.19500841002718422No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT32520.18829196835166362No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA32450.1878866658367615No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC32320.18713396116622905No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA31750.18383364068774047No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC30420.1761328929046005No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT28430.16461072140952634No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT27890.16148410200885296No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG27700.16038399518269011No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT26700.1545939592555172No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT26660.15436235781843025No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA26270.15210424380683282No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC26100.15111993769921342No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT26090.1510620373399417No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA25690.14874602296907252No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA25200.14590890536475778No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT25150.14561940356839914No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT24880.14405609386806245No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC24850.14388239279024725No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG24370.14110317554520427No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC24230.14029257051540006No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT24170.13994516835976967No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA23930.13855555973724817No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG23750.13751335327035705No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT23730.1373975525518136No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT23700.1372238514739984No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC23600.1366448478812811No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA23590.13658694752200937No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT23380.13537103997730307No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA23350.13519733889948787No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT23250.1346183353067706No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG22310.12917570153522803No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC22260.1288861997388694No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT22080.12784399327197826No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC22000.12738079039780442No Hit
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG21790.1261648828530981No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC21720.12575958033819598No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT21320.12344356596732683No Hit
TAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGA20970.1214170533928163No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGGAATAG20950.12130125267427284No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT20800.1204327472851969No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC20560.11904313866267541No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT20510.11875363686631676No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA20420.1182325336328712No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA20080.11626392141763241No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG20020.11591651926200201No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC19990.11574281818418683No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT19710.11412160812457842No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG19670.11389000668749148No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG19590.11342680381331767No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC19450.11261619878351345No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA19390.11226879662788308No Hit
GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG19090.11053178584973121No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG18960.10977908117919871No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT18920.1095474797421118No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA18720.10838947255667722No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT18660.10804207040104682No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG18550.10740516644905782No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG18490.10705776429342745No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT18480.10699986393415571No Hit
TTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAG18450.10682616285634051No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA18440.10676826249706879No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC18440.10676826249706879No Hit
GTCTAGAATTGACCTGCATGGTACCCGGGCATGCTCGAGCTAGCAGATCT18340.10618925890435149No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG18140.10503125171891692No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT18120.10491545100037346No Hit
GTGGAATAGGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCT18000.10422064668911271No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG17960.10398904525202579No Hit
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTT17940.10387324453348235No Hit
GTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTGTA17940.10387324453348235No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA17930.1038153441742106No Hit
GTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC17930.1038153441742106No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC17920.10375744381493889No Hit
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT17850.10335214130003678No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA17830.1032363405814933No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT17820.10317844022222158No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT17780.10294683878513466No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT17730.10265733698877602No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA17670.10230993483314563No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA17480.10120982800698279No Hit
GCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAA17410.10080452549208069No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT17310.10022552189936339No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAG100.007024771145.4579145
TGTATCG100.007024771145.4579145
TCTCGGG253.6434358E-8145.4579145
TTGCGCG100.007024771145.4579145
GATTAGG100.007024771145.4579145
CGTTCGA100.007097647144.956659
TTTCGCG450.0129.29593145
CTTAGGG351.8626451E-9124.67821145
TCCTCAG650.0123.07977145
ACCCTAG301.0815165E-7121.21493145
CCGTTCG301.0815165E-7121.21493145
AATCGCG256.3656425E-6116.36632145
GCATACG458.185452E-11113.133934145
CCGTTCC1050.0110.82507145
ACTCTGG203.818342E-4109.09343145
CTCTTAG203.818342E-4109.09343145
CCGTATG404.7130015E-9109.09343145
CGAGACG203.818342E-4109.09343145
TCGCCGG553.6379788E-12105.78757145
CTCTTGG553.6379788E-12105.78757145
Produced by FastQC (version 0.11.5)