FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_H11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_H11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871631
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT12540.14386821946442932No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT9120.10463143233776677No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC8910.10222215593525241No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG8720.1000423344282156No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG301.5750024E-596.965645145
CGACGAG353.3847784E-583.11341145
GGTCGCG607.860399E-872.724236145
GCCGTAG851.6552804E-1068.44634145
TCGCCGG451.1756663E-464.64376145
TAGATCG350.003522981762.33506145
AGCGAAA2000.054.3678741
TATACTG4250.052.8632855
GCTATAT2050.049.505711
ATACACG951.880182E-645.9311145
GCGAAAG2600.041.8142432
CGAAAGC2900.039.9878733
TTATACG800.00200583236.362118145
AGATGTA6000.035.0310447
AATATAC5700.034.3316963
TATAGAC4300.033.710712
GTAGAAA6150.033.0038071
GTATAGA4500.032.2181
CATTAAG7000.032.0974122
GTATTGT1402.7487316E-531.0673581
Produced by FastQC (version 0.11.5)