FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_H10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_H10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882024
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAAGGAGAGGGAGGGAAAGAAATGGGAAAATAGCATTCTAAAACTC22840.2589498698448115No Hit
GTTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTAC17610.19965443117194087No Hit
ATTATTAACTGTACTGACTTCAAGGCACTTGATTTCCCTTATTGGTTAAT17330.19647991437874707No Hit
GACCCACACAGTGTGAAGTGAGTACCTTCCCTCATTTCCCTCAGCTGTAA16410.18604935920111018No Hit
CCTCTATGATGCGCAGCAGGGCCTTGACGCCGGGTCTCCACAGGTGGCGC14980.1698366484358702No Hit
GTGTGAAGTGAGTACCTTCCCTCATTTCCCTCAGCTGTAATATTACCCTG13140.14897553808059644No Hit
ATACAGGACTGGTGTTCCCATTATTCAAAAAAAGTAAACCAAGAGATGTA12010.1361640953080642No Hit
GTGTTGAAAGTGTCTGAGCATTTCCTGGGTTTCCTGATGGATCAAAAGCT11980.1358239685087934No Hit
CATGTATTCCATCAACATTCTTTGTTTTTGATTAACTGATGAATAAGGAA11710.13276282731535652No Hit
TTATTGGACTGGGGGCCGGAATTGGAACAGGGATTGGGGCCGGAATTACA11600.13151569571803035No Hit
ATTAATACTCTGCTTACCCTGATGTAGAACTAATATGCTTATTTTGTGCC11170.12664054492848267No Hit
ACATTCTGGAGACCTGGAGGCCCCCGCAGGTCCTGCAGGACTACTACGCG10410.11802399934695655No Hit
GATCTGGACCAAGCCAAGGAGGAGGTGTTCCGCCTACCCCAAGAGGAGGA10050.11394247775570732No Hit
TCTTAATAGTTGGTATGAAAGTGCCACATTTTAAGGAGGAAAAGAGAGAG9930.11258197055862426No Hit
GAGGAAGACAGATAATAGTTTAAAGGAGAGGGAGGGAAAGAAATGGGAAA9820.11133483896129812No Hit
GAATTACAGCAGGAGCTGAAGCTGGAGTGGGTCGAGGCCTAGGTGCTGGC9750.11054120976299964No Hit
GTGCTGACATCTACTTGGCTTTTCATCTCGCTCTGGCGAGGGTGGGGACA9490.10759344416931965No Hit
ATTTTAAAGTACCCTCGTTCATTTAGTCCTTACAATAACATGGTAGGATA9160.10385204937734122No Hit
GTAAGAAGGCCAAGGCCCCTGAAAGGCCAGGGACTCGCGTCAGTGAGCGT8860.10045078138463354No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAA100.0070294193145.4217145
GCGGACG100.0070294193145.4217145
AGCGAAA4800.0117.846671
GCGAAAG5350.0105.68362
CGGGCCA353.445657E-582.815389
AAGGGCG300.001913492172.71085145
CGGGTAG502.2665627E-672.710846145
GGTCGCG502.2665627E-672.710846145
CGAAAGC9250.061.1251143
TGTCTCG606.6887387E-660.592377145
TATATAA1950.059.477432
GCTCTAG502.0072334E-458.0168231
GGCTAAT502.0072334E-458.0168231
CTAATGC502.0117423E-457.990493
ACGCTGG651.0748849E-555.931423145
CTAGGAG400.00605457354.366093
TATTGGA6200.045.5973663
TATATAT3850.043.304592
GGATACT2050.042.4200364
ATATAAT2850.040.695083
Produced by FastQC (version 0.11.5)