FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_H06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_H06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436093
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG406.011578E-790.89461145
TGACGCG300.001913233972.71568145
AGCGAAA6200.063.158641
ACTACGC350.003571684762.1214833
GCGAAAG6800.057.5617452
CGAAAGC10150.041.4143183
GTTCGTC700.001058948241.4114347
CTTGCCG1001.3676407E-436.357845145
TATACTG11550.035.13335
TAGGGCG850.002702727234.219147145
CTAGTAC4250.034.105913
GTAGGCG1501.1374032E-633.8216933
TTCGACA900.003638595332.2044074
ACCGTGC1652.3944485E-630.7459268
GACGACC1203.9888814E-430.298203145
GTACTAG4800.030.214781
TAGATCG1100.00953390126.442068145
TTATACT14650.025.719564
ATACTGG14050.025.272486
CCTTATA11000.024.3813722
Produced by FastQC (version 0.11.5)