FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_H05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_H05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307935
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT63150.4828221585935081No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT41810.3196642034963511No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA22770.17409122012944067No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG21540.16468708307370014No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA20850.15941159155462617No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT20400.15597105360740404No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA20270.15497712042265097No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA19610.1499309981000585No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG19540.14939580330826838No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG17540.1341045235428366No Hit
CCCTTTACAGGAAATGTGACAAAAATCTTCAGAATTGCTGAAATCGCTGA16730.12791155523783673No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC16690.12760572964252812No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA16070.12286543291524425No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT16000.12233023812345414No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC15560.11896615657505916No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC15140.11575498782431849No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT14620.11177925508530623No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA13610.10405715880376319No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT13540.10352196401197307No Hit
GTACATGTCTGGCACCTGATAAGAGCTGATAACTGTAGATGGGTTTTGTT13300.10168701044012125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3500.060.0912171
TGACGCG701.6651715E-551.947903145
ATGAACG2300.050.4070246
CAAGGAC17950.048.4394383
GGACAGT20750.045.3966876
GCGAAAG4650.045.200542
TAGATCG700.001040625541.558323145
GTAGAAA9450.041.442221
GACAGTG22850.040.9090167
CGAAAGC5150.040.8012163
CCGACGG1303.6099118E-739.16073145
ACAAGGA25100.034.650232
AGGACAG28900.034.6003235
GGTGACC14850.030.7441738
AAGGACA28250.030.266524
TCGCCGG1000.005983013729.09083145
CTATGCG2303.508103E-828.3539524
CTTATGA4500.027.3727683
AGTAGAA9150.026.9487671
GAACCGA1100.00969864126.3491296
Produced by FastQC (version 0.11.5)