FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_H03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_H03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640026
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA38260.23328898444292956No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT26270.16018038738410245No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT26210.15981453952559288No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG25680.15658288344209179No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT19490.11883957937252215No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA17460.10646172682628202No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC17310.10554710718000812No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG16700.1018276539518276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG750.087.26318145
AGTAGAA10650.063.9922521
GTCGCAT1206.002665E-1154.344893
ATACACG1152.421075E-950.587353145
GTAGAAA13600.050.099352
GTACGAC657.3370326E-444.6165281
ATGAACG2600.044.596126
CGCATCG1601.0149961E-940.7623985
CCGTATG1105.158232E-639.66508145
GGTCGCA1651.369699E-939.536822
CAATTCG2550.036.949339
TCGCATC1854.2073225E-935.2518124
ACTAGTA1051.8566131E-434.516272
CCGACGG1501.1080028E-633.935684145
CCTTATA17050.033.5850372
TAGAAAC22950.033.4672973
TTATACG1551.4315847E-632.840984145
CGAAAGC5300.032.8120083
CAGGTAC9400.032.375683
CGAACGT900.003634414632.212249
Produced by FastQC (version 0.11.5)