FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_H02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_H02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921692
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACGGACCCCAGGTATCCGGCCGCCAGAATCAACATGGCTGTCATG50710.5501837924165557No Hit
ATTCTAGGGAGTTGCATGAGAAAAATGAAAACCTAGATCCCAACGAGGCT28980.3144217374133658No Hit
GGTATAAACTACGGGCGCAACAGGGCAGAGGAATCTCCAAGACCTCCCGC23070.2503005342348637No Hit
ATAAAAGACAGGCTATCATCATCAGTTGCCCACAACCATGTGCAAATCAC22230.2411868606866502No Hit
GGATAAGAGAAGGAATGTGGGAGCTTGTGTTTGAACTCCATGCTTTTGAC18850.20451517426645777No Hit
CTTCTAGGAGAGGATGAAGGGAAAAACCCAGAGCAGTGACTGCCTGGGAT18720.20310472478875805No Hit
TCTCAAGGTAATCCAACCTTTCCTTTTCAGTTGAACTGCTGCAAATAACT17630.19127864839881434No Hit
CTACAAACATCACTAAACACACGCACACAAACACCCTTCAACAAAGTAAC17600.19095316005780671No Hit
GTAGTAAACAGGTATAAACTACGGGCGCAACAGGGCAGAGGAATCTCCAA17380.18856624555708415No Hit
GTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGACCTAATGTCC17040.18487737769233106No Hit
GAGTAGGGGGTGCTGGTATGAAAGACTGTGAGTAGAAAGGAAGATGGGAG16940.18379241655563897No Hit
CCCTATACTCTTCCGGGACACTGGGTTGCAAGCCACACCTATGCTCAATT16780.18205647873693165No Hit
GTCTGTGATCTGGTGTTTTTTTTACTCAAACAGCCCATGCGGTGGATCCC16660.18075452537290113No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT15800.1714238595973492No Hit
CCCTAATGGTTATCGACATTGCTCCGGAGCAGTAGACCCTATCAAGGACA15780.1712068673700108No Hit
AAGCAGGGGCGTGAAGAAAGGCGTGATGGATGTCCTTAGGTTTGTTTACT15750.1708813790290032No Hit
GTGCCAGGAGAGCGGGTCACCGGAGCATGGTGGGTGCTTTTGCTTTCGGT15460.16773499173259612No Hit
ATCAAGGACAGCTATTGATCGCTTGTGTTTTGATTAGATTGGAAAACAGA15160.16448010832251989No Hit
AAGCAGGGGTCATTGAAGGCACTCGGCAAGGCAGGGATAAGAGAAGGAAT15140.16426311609518146No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT14930.1619846977081281No Hit
ACATTGATCCGAGCACACACCAGGCCAGCAGATGTGCACATGCGCGTGCA14310.15525793866063717No Hit
ACTTATAACCTTCCAAAGCAGAATTTATTTCTGCAAAAGTGATTTGAGGA14120.1531965125009222No Hit
CTCCAAGACCTCCCGCTGTGCCAGGAGAGCGGGTCACCGGAGCATGGTGG13540.14690373790810812No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC12870.13963449829227118No Hit
GTGTAGAGCCCTGAGTCCTCACTGAAGCCCGAGAGGAAAGATGGATGGTG12750.13833254492824068No Hit
GAATATAATTATGGAAAATTTCAAACGTATACAAAGTAAACAGAGCAGTA12670.137464576018887No Hit
CTTTTAAAGTGACTCATTAACTTCTTATCTTTGTTTTAACTTATAACCTT12570.1363796148821949No Hit
GTATGAAAGACTGTGAGTAGAAAGGAAGATGGGAGGAGAGAGTGTGAAAA12330.13377570815413392No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC12310.1335587159267955No Hit
GCTATTGATCGCTTGTGTTTTGATTAGATTGGAAAACAGATCAACTTCAT12140.13171428199441898No Hit
AGTTTTAACAGTAGCTTTATTCACAGCAGCCTCCAACCCAAACCAGTAAA11980.12997834417571164No Hit
CTTCTTATCTTTGTTTTAACTTATAACCTTCCAAAGCAGAATTTATTTCT11950.12965285583470398No Hit
GTTCTATTCTAGGGAGTTGCATGAGAAAAATGAAAACCTAGATCCCAACG11850.12856789469801191No Hit
GATCATGACAAGAGGACAATGGTTGTTGGCCACGTGTCTGGCGCTGGGCC11830.12835090247067352No Hit
GAGTAGGGAGGAGGATAATGTGTGGGTCTGCACCCGTGGAAAGGGAAATT11720.12715744522031222No Hit
GGTTAGAAGCAAGGGCGATCTCCCAGTCGTTACACCTCTTGAGGTAAGAT11660.12650646853829695No Hit
GAGAAGGAATGTGGGAGCTTGTGTTTGAACTCCATGCTTTTGACACCTGC11330.12292609678721308No Hit
CCTTTATTAAATATCCATAAAAACAGGCTACAATAAAAGACAGGCTATCA11310.12270910455987466No Hit
CAATAAAAGACAGGCTATCATCATCAGTTGCCCACAACCATGTGCAAATC11230.12184113565052102No Hit
ATTATATTCTTTTTAAGTAAAAGAGCTTTTAAAGTGACTCATTAACTTCT11200.12151564730951338No Hit
GTGGTAAGATTTGTACCCTTTTTTAAAAGAGCAGCTTTATCGAGAAGCGA10810.11728429887641426No Hit
GTGATGGGACGTCAGGAAAACCGTGTGCCAAGTCAAGACCAGACCAGACC10800.11717580276274504No Hit
GGTCTGACAGGACCAGAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGG10740.11652482608072978No Hit
CACTACCACTGTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGA10720.11630783385339137No Hit
CTTATAACCTTCCAAAGCAGAATTTATTTCTGCAAAAGTGATTTGAGGAC10700.11609084162605295No Hit
ATTAAATATCCATAAAAACAGGCTACAATAAAAGACAGGCTATCATCATC10670.11576535328504532No Hit
GGATAACATTGATCCGAGCACACACCAGGCCAGCAGATGTGCACATGCGC10640.1154398649440377No Hit
GAGCAGTATAACGGACCCCAGGTATCCGGCCGCCAGAATCAACATGGCTG10390.1127274621023075No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG10350.11229347764763066No Hit
GTGTGGGGGCTGTCTGTGATCTGGTGTTTTTTTTACTCAAACAGCCCATG10270.111425508738277No Hit
GCCCACAACCATGTGCAAATCACACTCTACAAACATCACTAAACACACGC10150.11012355537424649No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA10020.10871310589654679No Hit
GCCTTGGGGAAGCCTGGTCGGAGAGCTGAGTTTCCTCGCGAGCCGGCAAG9930.10773664087352391No Hit
GAGTGAGGAGGCGGTCTCGCCAGGTCCTCTTGGGGCGGTGGAGGGCGCCC9890.10730265641884708No Hit
GATTGAAAGAGTAGTGAAGAGAGGAGTGGCAAGTGGATAAAAGAGGAAGA9810.1064346875094934No Hit
TCTTACCACAGAGCGACAACTGAAAGAAATCCAACACAGTTAATGTGCGT9670.10491574191812449No Hit
CTCCTAGAAGTTCGTACTAAATGGAACCACCGAAAGCAAAAGCACCCACC9620.10437326134977847No Hit
GTGGTGAGGAGGGTGTGGGGGCTGTCTGTGATCTGGTGTTTTTTTTACTC9620.10437326134977847No Hit
GTCAAAAGCATGGAGTTCCTTTATTAAATATCCATAAAAACAGGCTACAA9590.10404777300877083No Hit
GTATATGTTCTAACAACAAGCAGACTGGTTATTGAAGTGTTACTTTGTTG9560.1037222846677632No Hit
GTTCATTTCTTGTGCGTCTCCAACGGGCTGCTTTCCCACTTGCTTGTCCG9540.10350529244042479No Hit
AAACAGAGCAGTATAACGGACCCCAGGTATCCGGCCGCCAGAATCAACAT9530.10339679632675558No Hit
AAGCAGGACAGGGGCAGAACGGGTGTGTATCTATTTTTAAAAAAGTCCAC9500.10307130798574796No Hit
GATAAAAACCAGCTCGAGAATCTTGACTAGCCTGCTAGCAACTGCAGAGG9490.10296281187207874No Hit
ATGTGTGGGTCTGCACCCGTGGAAAGGGAAATTTTCCATGTCTGTCAATC9390.10187785073538665No Hit
GTAGTGAAGAGAGGAGTGGCAAGTGGATAAAAGAGGAAGAGAAGGAGGGG9360.10155236239437902No Hit
GGTGAGGAGGGTGTGGGGGCTGTCTGTGATCTGGTGTTTTTTTTACTCAA9340.10133537016704061No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGC9310.101009881826033No Hit
GCCATGAAGGAGTCTTTTAGGTGACAGAAATGTTCTAGCTCTTAATTACA9230.10014191291667933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTCG306.366463E-10145.42104145
TTGTCGG256.3720763E-6116.33683145
TTCCCCG256.3720763E-6116.33683145
CTTCCCG203.821621E-4109.06578145
CTAGCCG501.9099389E-10101.79472145
AGCGAAA7850.099.757881
CACATTG406.0123966E-790.888145145
GGTCAAG406.0123966E-790.888145145
ATTTGCG259.279007E-487.252625145
GCCGTAA259.279007E-487.252625145
TTCTCGG259.279007E-487.252625145
GCGAAAG9350.083.726672
TTATACG554.3066393E-879.32057145
AGCAACG406.567872E-572.71052145
TAGATCG300.001913554372.71052145
TGAATTG607.871313E-872.71052145
TGACGCG350.003525680662.323303145
TCGAAAC350.00356922462.130512
CGAAACC350.003572294762.117023
GCCGCGG606.689159E-660.5921145
Produced by FastQC (version 0.11.5)