FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_G11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_G11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089615
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC17520.1607907380129679No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT15620.14335338628781727No Hit
TTCTAGTACTGTGCCACTATTGAATTGAATAAGTTCATTGTTGTTTAAAT14910.13683732327473466No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG12910.11848221619562872No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC12410.11389343942585226No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG11590.10636784552341882No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC11130.10214617089522446No Hit
ATTTTATAGAGGTGGAAACCGAGGCGCAGAAAAGTGAAACAACTGACTTG10920.10021888465191833No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG203.81685E-4109.10139145
CGTTACA259.403939E-486.960469
TCGTTAC259.405654E-486.956478
TGACGCG451.2095297E-680.81585145
CCGACGG451.2095297E-680.81585145
AGCGAAA2900.067.500311
GTCGTAG651.0936787E-555.7694931
CGAAAGC3650.053.615613
ATACACG858.7290937E-751.34183145
GCTATAT2450.050.3063621
GCGAAAG3900.050.1833232
CATTAAG6900.048.3246842
CTATATA3600.040.270572
ATATACC3500.037.2704774
GTAGAAA7650.036.013241
TATATAC5200.034.846333
ACCGTAC850.002742027234.117811
TAAGATG8100.033.9968875
GTACTGT9800.031.7938486
TTCAGCG1153.1030094E-431.62359145
Produced by FastQC (version 0.11.5)