FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_G10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_G10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080623
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAGGTTCTGAGAAATCATACAATAGAAAAACCTTTTTACCTTGGG44920.4156861366082344No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT29130.26956672215934696No Hit
CTTATAAGGAAAGGAATTAAATCTTGCTATTTGGTTAATTTTCACCCTCA24410.22588821448368213No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG20310.18794713790100712No Hit
CTATAATGTTTTCATTGATTTTGTTTTTTGCTATGAGTTCTGTAATTGTT20300.18785459868982984No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGC18780.17378863859088692No Hit
GTATAGAGGCATGAATTTGATGGGAGTTTATTTGGCTCCATTCTTACTAC18570.17184531515616455No Hit
TCTCAAATCTGTGTGAACCATGAAATATCTAAACACATTCTACGGAACTA17270.15981521770312127No Hit
ATACATCACTGAATAAAGGTTCTGAGAAATCATACAATAGAAAAACCTTT16800.1554658747777902No Hit
GTCCTGGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGC16580.1534300121318906No Hit
GCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCC16570.15333747292071334No Hit
CTTTTATATTGGCCATAGCAACTTCTTTCAAGACATGTCTCTAAAGGCAA16310.15093145343010467No Hit
GGTCTGGGTCCTGTCCATCAGGATTTTCACAGACTTGGTGTTCTTCATGA15450.14297308126886066No Hit
TCTATGGATAGAGAAAAGTCTCATTCCATCGAATTCAAATGTGCACTTGG15280.14139991467884727No Hit
AAATAATACCTGCCATATTGGGGTCTAACTTTTAAAAGTGAGAGCTCTAT14900.13788342465411157No Hit
GTGGAGGAGAAGGTGAGCCAGCTGAAGGAGGAGGTGCGGCTACAGTATGA14820.13714311096469353No Hit
AAAAATACCAGTATCTCAAGAAAGCATGCCACTTAAACAAAAATCTCAAA14800.13695803254233901No Hit
GCTTAATTGTATACCAATGTGTACAGGAACATCTATGGATAGAGAAAAGT14750.1364953364864527No Hit
GTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGC14320.1325161504058307No Hit
ATGTAGCAGGGTCCCATATGAAGAACTGTATTTTGTATAGAGGCATGAAT14170.13112806223817186No Hit
CAAGAAAGCATGCCACTTAAACAAAAATCTCAAAAATGCACCAAACAGAA14100.1304802877599311No Hit
TTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGCCACCTCATTCAGGA13670.12650110167930906No Hit
GTAGATGTACTGTGCACGCGGGGAGCGGTGGCGATCAGTTACGGGGAACA13650.12631602325695457No Hit
CTCTTAAAGATTATACATCACTGAATAAAGGTTCTGAGAAATCATACAAT13610.12594586641224553No Hit
ACCTATAGCTTAATTGTATACCAATGTGTACAGGAACATCTATGGATAGA13290.12298461165457333No Hit
CTCTTACATCATACGCAAAGATAAACTCAAAATGGATTAGAGACCTCCAT12480.1154889355492156No Hit
ATTGTATACCAATGTGTACAGGAACATCTATGGATAGAGAAAAGTCTCAT12120.11215752394683437No Hit
GTATAGGGTAGAGTGAAAAGAATCAGAGATAGGAACTGTATGCCACTATC11900.11012166130093475No Hit
GGTGTAAGGTGGTATCTCAATGTGGTTTTGATTTGAATTTCCCTGATGGC11200.10364391651852681No Hit
GTACAGGAACATCTATGGATAGAGAAAAGTCTCATTCCATCGAATTCAAA11110.1028110636179315No Hit
AAGCAGGACATCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCT11090.10262598519557699No Hit
GCCATATTGGGGTCTAACTTTTAAAAGTGAGAGCTCTATAATGTTTTCAT10820.10012742649379107No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG100.00702249145.47119145
GCTTGCG100.00702249145.47119145
CCGAACG100.00702249145.47119145
TGCGTTA450.0129.30772145
ACACTCG301.0806616E-7121.226145
CCGACGG203.8165617E-4109.1034145
AGCGAAA6700.085.508641
GCGAAAG7100.079.65142
CTTATAG554.2969987E-879.34792145
CCTGTCG502.2623299E-672.7356145
TGACGCG702.741217E-972.7356145
TTACTCG406.5571716E-572.735596145
TATAAGG5900.068.797483
TTATACG754.7330104E-967.88656145
CTGTTCG350.003521030362.344803145
TTATAAG7000.058.0025562
TAGCTAG400.005973843454.5517145
CTTATAA7700.052.7418061
GCGTCCC701.6993545E-551.768829
AGCGTCC752.5556461E-548.3175668
Produced by FastQC (version 0.11.5)