FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_G09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_G09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091058
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG400.006062092754.351664
AGCGAAA5800.042.5163351
CAATTCG2650.041.0201239
CGAAAGC7300.033.7526323
AACGCGG1000.006090132528.9861665
GCGAAAG10150.024.2840042
TATAGAC17450.023.680282
TATCACG1902.2006696E-422.8849097
GTATATA21600.022.1612911
GTATTAC12750.021.6161481
TATATAC23350.021.1044273
ATAGACC10350.021.0054743
ACTATAT8700.020.8243923
TAGTACC4551.546141E-1020.7053954
CCTTATA8100.020.584972
TATACCG1800.00432053320.1292825
GTATATG11250.019.9854551
TATACTG14300.019.2565445
TATAATA11000.019.1122882
CAGCTCG11450.018.9874789
Produced by FastQC (version 0.11.5)