FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_G08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_G08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184113
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6000.055.590021
GTCGCGT700.001056291241.433551
CGAAAGC8950.038.058023
GCGAAAG9150.036.4441222
TGACGCG1209.379413E-636.35598145
TAGATCG1707.63157E-834.217392145
GCGCACG2451.9135769E-929.678349145
GTAGAAA19500.029.3753261
GTACACC15600.026.944793
CTATACG2456.438313E-826.6358551
CGTACCG1100.00969215326.3535235
GCATTAA11100.026.1292651
CCGTCCG1801.5243431E-424.1590567
ACCGTGC10050.023.7984738
GCTATTG12950.022.946448
TTGCACG3503.3905962E-922.85233145
TACACCC17550.022.7126034
GAGCTAT14100.022.616476
CTATTGA13200.021.9627789
TGTCTCG2704.337686E-621.544285145
Produced by FastQC (version 0.11.5)