FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_G07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_G07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341789
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT37560.27992478698215595No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC36990.2756767271158133No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC30650.22842637702351112No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC28450.21203035648675014No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG27630.20591911246850286No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC22310.16627055371597174No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT20390.15196129942934394No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG18880.1407076671518398No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA17840.13295682107991644No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA17790.1325841842495355No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG17010.12677104969559297No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG16610.12378995505254553No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA15240.11357970590010799No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA15070.11231274067681284No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA14950.11141841228389858No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT13840.10314587464944192No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG301.5758309E-596.95924145
TGACGCG753.6844176E-758.175552145
AGCGAAA2900.055.028561
GCGAAAG2900.055.012142
GCATTAA11500.049.830211
TAGATCG803.6692552E-545.44965145
CGAAAGC3450.044.122073
CGAATTG907.351667E-540.399693145
GTAGAAA14200.039.8446241
CATTAAG15550.038.2399522
GATTAGC7500.034.8180731
TTAGCAC8750.034.7934043
GTATAGG6050.034.7701151
AGATGTA14900.034.5327077
TAGCACA8750.031.4738794
TGTCTCG2601.07320375E-1030.765915145
TAAGATG19150.030.2735655
TTAAGAT19500.028.9891014
ATTAGCA9450.028.3925082
TAGAAAC19750.028.2720622
Produced by FastQC (version 0.11.5)