FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_G06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_G06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881952
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC16440.18640470229672362No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC13050.14796723631218026No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT12420.1408239904212474No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA12150.13776259932513332No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA12140.1376492144697217No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT12130.13753582961431007No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT12040.13651536591560537No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG11330.1284650411813795No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC11250.12755796233808642No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC10920.12381626210950256No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC9880.1120242371466928No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA8870.10057236675011791No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGTA450.00953190348.469906145
ACGAATA450.00953190348.469906145
AGCGAAA2000.047.1535531
GCGAAAG2600.039.0554662
GTAGAAA8850.038.5261541
ATGAACG1905.4442353E-934.3297926
CGAAAGC2800.033.6581843
TATAGGG4200.031.0849632
GTATAGG4000.030.831171
CCATCGG2859.094947E-1230.5170979
AGTAGAA7550.029.786251
GTTCGGT1255.13511E-429.0175721
TAGAAAC12600.028.7823732
ACCATCG3600.028.1859328
TATACCC2951.43372745E-824.5660675
GCATTAA6000.024.181311
TCGGTCC1550.0018091123.385343
TGAACGT2802.3500797E-723.2952147
GAACGTC2852.786128E-722.8878238
ACCGTGC6350.022.8277518
Produced by FastQC (version 0.11.5)