FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485582
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA56710.38173591225526426No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC26040.175284837861525No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG24100.16222598281346973No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG21230.14290695498464576No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC20470.13779111486272722No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG19810.13334841159895583No Hit
GTTTAAGACCTCTCCACAAAAGACAATCAACTTGCTCACACCTTATATAC17660.11887596914879153No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT17470.11759700911831188No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG16730.11261579636802277No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTATCTCAAATCGGTGCTTC16130.1085769752191397No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC15310.10305725298233284No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCG100.0070258514145.44975145
TGACGCG501.9099389E-10101.81483145
CCGACGG851.274384E-859.891068145
CGAAAGC4850.049.3096353
AGCGAAA5250.045.577261
GCGAAAG5350.044.7133032
GTAGAAA13650.043.0274851
CAATTCG2151.2732926E-1137.0790379
ATGAACG8250.036.893936
CTATACG1251.2643906E-534.8044551
GGTCGCG850.002701085334.223465145
TAAGACC12750.032.3973584
TACTGAT11800.031.9371037
CTAGTAC4600.029.933353
TACTATA3400.029.8487192
TAGAAAC19650.029.5123652
TTAAGAC12950.029.1000123
TGAACGT10500.028.9890637
GTGATCA29050.028.9391699
AGTGATC29100.028.1423038
Produced by FastQC (version 0.11.5)