FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_F12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_F12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080445
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAATGGGCGCTGTGCTGCCAACATAGCAGCTGTAGCCGGGACTGT66580.616227572898204No Hit
GTCGTGTAGTGTGCGCGGATTCCACCAGAGAAGGCCTCCAGGATAAAATA54660.5059026604778587No Hit
ATCATATGGAGAGAAATTATCCTATTGTCCGTTTCCAGTGAACACTGCAG36200.33504713335708897No Hit
ATATAAACTGTCTGCAAACAATCCACTCTCCCTTGGTTTTCTTGGTCTCC34350.31792455886232057No Hit
CTACACGACAGTCCCGGCTACAGCTGCTATGTTGGCAGCACAGCGCCCAT32590.30163497447810855No Hit
CACACACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGC31930.295526380334029No Hit
GAATAGAAAGAGGGATGTGTTGCAGGAGTGGAGGGGAGAGAAGAGAAAGA26940.24934170642651868No Hit
GTAGAGAAGAGAGAAGAGAAAGAGAGGGATGCGTTGCAGGAGTGGAGGGG26030.24091925086422725No Hit
GCTTATAATACTTAATGTATACCAGCCTTTTTACCCCTAATATAAAAAAG24800.2295350526866245No Hit
CACACACACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTG24220.2241668941963728No Hit
GCCATAGACAGAGCATTCCCTTTTTCCCAGATGGATGTACGCAGAAGAGA24100.22305624071563107No Hit
GTGTGGTGCTGGCAGTGTGCCTCAGTTTACAACTTACAGCTCCCCGTGTT23710.21944661690322045No Hit
GATTCATACACGTACAGAAATTAAATATACAGAATTGGATAAAAATGGAG23470.21722530994173697No Hit
GATTTACTGCTATCTGAAATAGAGACATTCATCAGGCACATGGAGTACCT22770.21074649797074352No Hit
GGCACATGGAGTACCTTTTGTCCTCGGGCCTACGCGTTCAGTCCCCAAAA22650.20963584449000178No Hit
GCTCTATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACT21980.2034346958891938No Hit
ATCTGAAATAGAGACATTCATCAGGCACATGGAGTACCTTTTGTCCTCGG21480.19880697305276995No Hit
CAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGCGCTGATGA20060.1856642401973261No Hit
ATGTATACCAGCCTTTTTACCCCTAATATAAAAAAGCATCTGCTAAATGA19790.1831652698656572No Hit
ATGTATATGTAAATAGTTTTGTGCTTCATTGCTCTGCTTATGCTTAGTAA19490.18038863616380288No Hit
GGGCATAGTTGACCCCTTATTACGACATGGTATTAAATGGGCGCTGTGCT18620.17233639842842532No Hit
ATACAGAATTGGATAAAAATGGAGAAAGCCAGCATTCAAAGTACTGGTTG18510.17131829940441207No Hit
GAAATAGAGACATTCATCAGGCACATGGAGTACCTTTTGTCCTCGGGCCT18470.17094808157749816No Hit
CTGCAGAGTGGGACGAAGGCCATAGACAGAGCATTCCCTTTTTCCCAGAT17630.16317350721230603No Hit
TTACAGGACAAGGTAACATTCAGTCCTTCCTCTGTAAAGACTTCGGTTCT17490.16187774481810735No Hit
GATATATCATTTATCAGGTACATTCAATTTGTGTTGTTGATGAACATAGA17330.1603968735104517No Hit
GTGTTGCAGGAGTGGAGGGGAGAGAAGAGAAAGAGAGGGATGTGTTGCAG17210.15928622002971No Hit
CATCTGGTTTGACCACATGCTAGGTGAGGATCCTTTTGAGCCCAGTTAAA16820.15567659621729935No Hit
GTATTTAGGCTACATCTGGTTTGACCACATGCTAGGTGAGGATCCTTTTG16670.15428827936637218No Hit
GGACAGGAGAAGTGGCTCGATTAGATTGACTCTCAGAAATCAGATTTTCA16660.1541957249096437No Hit
CCTTTTGTCCTCGGGCCTACGCGTTCAGTCCCCAAAAAAAGGAAAGTAGT16530.1529925169721735No Hit
GATCTGGGATGCTGAATGTACCACTGCAGACTCTCTGCAGAAGAGTAATT16490.15262229914525957No Hit
GAGTACCTTTTGTCCTCGGGCCTACGCGTTCAGTCCCCAAAAAAAGGAAA16490.15262229914525957No Hit
TTGCAGGAGTGGAGGGGAGAGAATAGAAAGAGGGATGTGTTGCAGGAGTG16440.1521595268616172No Hit
CATATGGACTGTGGCAATGTAGAACGTGGATCCTTTACCATTGGGCCTGC16420.15197441794816025No Hit
ATTTAATACCATGTCGTAATAAGGGGTCAACTATGCCCAAAGTATTTGAG16360.15141909120778937No Hit
GGCCGACGGGGGAAGAAGGATTTACTGCTATCTGAAATAGAGACATTCAT16330.15114142783760395No Hit
GTGCTGGCAGTGTGCCTCAGTTTACAACTTACAGCTCCCCGTGTTAGCGG16310.150956318924147No Hit
GATTATTAATGCGTGATGAGGTTGGTGAACTCATGGCACGGGATCTGAGA16190.14984566544340525No Hit
GAGTAGAGAAGAGAGAAGAGAAAGAGAGGGATGCGTTGCAGGAGTGGAGG16090.14892012087612047No Hit
GGCCTACGCGTTCAGTCCCCAAAAAAAGGAAAGTAGTGCTTTATTTGCCA15680.1451253881502529No Hit
GTGTTGCAGGAGTGGAGGGGAGAGGAGGCAGGTATTTAGGCTACATCTGG15630.1446626158666105No Hit
GTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGCGCTGATGATC15320.14179342770802772No Hit
TGCTAGGTGAGGATCCTTTTGAGCCCAGTTAAATCTCAAGTGCTCCCCTG15020.1390167940061734No Hit
GCTTATGCTTAGTAACCTTTTGAGGACACTGCTACCCACTAAGATGTTCT14860.13753592269851775No Hit
GCTTACAGCTGCCCATTCCCACCAAACTACTGTGCACAGATTCTACTCTG14740.136425269217776No Hit
GGAGAAAAGAGAATCAGGAGAAAGCAGATGGATTCATACACGTACAGAAA14360.13290819986209385No Hit
GAGAAAGAGAGGGATGCGTTGCAGGAGTGGAGGGGAGAGAATAGAAAGAG14160.13105711072752432No Hit
ACACTACGTTGTTCAGACGGATGTGTGCTAGCCATTAGCCTTGCACCTCT14120.1306868929006104No Hit
GGAGAAAGCAGATGGATTCATACACGTACAGAAATTAAATATACAGAATT14120.1306868929006104No Hit
ACTTACAGCTCCCCGTGTTAGCGGAGGAGTCGAGAGATTGAGAGAGAGAA14050.13003901170351106No Hit
GAATTGGATAAAAATGGAGAAAGCCAGCATTCAAAGTACTGGTTGTAGAA13760.12735493245838522No Hit
GTTTACAACTTACAGCTCCCCGTGTTAGCGGAGGAGTCGAGAGATTGAGA13690.12670705126128584No Hit
GATTCTACTCTGCTTATAATACTTAATGTATACCAGCCTTTTTACCCCTA13650.12633683343437196No Hit
GGCCATAGACAGAGCATTCCCTTTTTCCCAGATGGATGTACGCAGAAGAG13370.12374530864597458No Hit
CTCTGCAGAAGAGTAATTACAGGACAAGGTAACATTCAGTCCTTCCTCTG13330.12337509081906067No Hit
GGAGTAGAGAAGAGAGAAGAGAAAGAGAGGGATGCGTTGCAGGAGTGGAG13250.12263465516523285No Hit
ATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCATCT13110.12133889277103416No Hit
CCCCTGATAGACTCTTTATTTTATCCTGGAGGCCTTCTCTGGTGGAATCC13060.12087612048739178No Hit
GACTAAATGTATATGTAAATAGTTTTGTGCTTCATTGCTCTGCTTATGCT12940.11976546700665004No Hit
CTGAAATAGAGACATTCATCAGGCACATGGAGTACCTTTTGTCCTCGGGC12790.11837715015572288No Hit
ACTTAATGTATACCAGCCTTTTTACCCCTAATATAAAAAAGCATCTGCTA12780.1182845956989944No Hit
CACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGCGCTG12590.11652606102115332No Hit
GAGAAAGAGAGGGATGTGTTGCAGGAGTGGAGGGGAGAGGAGGCAGGTAT12560.11624839765096788No Hit
CTATACTGTACTCTAGTAGCCACCTCCACAATGTTGCTGTTTCAACTCAT12390.11467497188658377No Hit
ATCCTATTGTCCGTTTCCAGTGAACACTGCAGAGTGGGACGAAGGCCATA12370.11448986297312681No Hit
GTATACTGTTATTGTATTTGCTACAGTTATATGCCATTATGAGCTTGTCG12320.11402709068948443No Hit
CACACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGTTGGCGC12280.11365687286257052No Hit
CCTCTGGTGAGCGGGCGCAACAGCATCGGGCGGTTATACTCCTCTCCTCT12270.11356431840584204No Hit
GGGGTAAGGTAGATGGCGTGCAGTTTATTTAGTTTGATTGTTATGTGATT12240.1132866550356566No Hit
CCTTATTACGACATGGTATTAAATGGGCGCTGTGCTGCCAACATAGCAGC12140.11236111046837183No Hit
AGGTAACATTCAGTCCTTCCTCTGTAAAGACTTCGGTTCTGTCTGGAGTG12130.11226855601164334No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC12070.11171322927127247No Hit
CCTTTAAACCAGAGAAATGGAAACGATTTGAAGTAGAGAATGGTGTTTTA11910.11023235796361684No Hit
CATCAGGCACATGGAGTACCTTTTGTCCTCGGGCCTACGCGTTCAGTCCC11870.10986214013670294No Hit
CTCCAGTCCACCTCACCCCCCTTCACATGGGCCCACTTAACCCTTCCACC11860.10976958567997445No Hit
AAGCAGAACAGAATGACACGAAAGAGGAGAGGCGCAGCGGGCAGAGGAGG11850.10967703122324597No Hit
CACACACACACACAGTGAGAGTGAGAGTGCAGCTGTTGGATGGCGTGTGT11730.10856637774250424No Hit
GTTCAGACGGATGTGTGCTAGCCATTAGCCTTGCACCTCTCCGCTTGTAC11710.10838126882904729No Hit
TTGCAGGAGTGGAGGGGAGAGAAGAGAAAGAGAGGGATGTGTTGCAGGAG11550.10690039752139166No Hit
AAAGAGGAGAGGCGCAGCGGGCAGAGGAGGCTGTTAATCTATCACACGCC11440.1058822984973784No Hit
GGAGATAACGGCCCATAAAGGGCCCCATTGAGGGAGGGAGTGAAAAGGAA11430.10578974404064992No Hit
GAATGTACCACTGCAGACTCTCTGCAGAAGAGTAATTACAGGACAAGGTA11420.10569718958392144No Hit
GTCTGGAGTGATCTTGTTCCCCAAACCAACACCTTTAAACCAGAGAAATG11410.10560463512719297No Hit
CATATACATTTAGTCATTTAGCAGATGCTTTTTTATATTAGGGGTAAAAA11270.10430887273299427No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA11270.10430887273299427No Hit
GCTCTGGACTATCTCTGCTCGCAGTGCTTTCAAGCCTCTGGCAGACATTA11150.10319821925225253No Hit
ATGTACCACTGCAGACTCTCTGCAGAAGAGTAATTACAGGACAAGGTAAC11100.10273544696861014No Hit
GTTTCGGACTGGTGGTACACACAAAACCTTACAAATGAAACAGAGAAAAT11090.10264289251188169No Hit
GCCAAGGAGTGGCTGCGGACACAGGACAGGAGAAGTGGCTCGATTAGATT11030.10208756577151082No Hit
CATGCTAGGTGAGGATCCTTTTGAGCCCAGTTAAATCTCAAGTGCTCCCC10950.101347130117683No Hit
GGGGAAGAAGGATTTACTGCTATCTGAAATAGAGACATTCATCAGGCACA10940.10125457566095451No Hit
GGATTTACTGCTATCTGAAATAGAGACATTCATCAGGCACATGGAGTACC10940.10125457566095451No Hit
GTTATACTCCTCTCCTCTCTCATTATTACTCTCTCTCTCTCTCTCTTTCT10940.10125457566095451No Hit
GTGTGAGGCTGGCCTGATGGGGCCGCCCCGCTAACACGACACCTCGCAGC10910.10097691229076908No Hit
GTTGGATGGCGTGTGTTGGCGCTGATGATCAATGCGTGTTTGCTCTGCGA10810.1000513677234843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCGA100.0070271133145.43918145
GTTGCTT100.00709564144.967868
AGCGAAA7050.0117.2677841
GGTGTAC203.8199057E-4109.079384145
GCACTTT203.8199057E-4109.079384145
AGCGCCG406.0086313E-790.89949145
CTTAGGG406.0086313E-790.89949145
TTTAGGG1200.090.89948145
CGCCGTG651.4551915E-1189.50104145
ATTACGG259.274846E-487.263504145
CCATGCG259.274846E-487.263504145
CTGATCA703.092282E-1182.838789
GCGAAAG10100.081.8438642
TCATCCG451.210974E-680.799545145
ATCCTCG1550.079.756966145
TCTGTAG755.4569682E-1177.567566145
ACTGATC1050.075.935558
GCAGAAC2650.073.8515553
GCCGTAG502.2652912E-672.71959145
CCCCACT2200.072.520871
Produced by FastQC (version 0.11.5)