FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_F11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_F11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084846
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC30870.28455651769928636No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT26300.24243072288601333No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC20720.19099485088206067No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC20540.18933562920451383No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG19760.18214566860181078No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC15820.14582714965995172No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT15160.1397433368422799No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG15090.13909808396767837No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG14710.13559528264841278No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA14240.13126287049037375No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC13480.12425726785184256No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA12980.11964831874754574No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA12100.11153656832398331No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG11820.10895555682557709No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGCG851.6552804E-1068.42647145
AGCGAAA2200.065.926051
CATACCG350.00357272762.115875
TGACGCG606.693994E-660.58594145
GGTCGTT901.3272274E-648.3145687
GCGAAAG3250.044.606292
CGAAAGC3550.040.8329773
GCATTAA11750.040.7338871
TAAGATG11350.038.9478385
TGGTCGG951.0121344E-438.2648145
CGCACAA1553.3862307E-837.524197145
GTAGAAA11150.035.7715381
CATTAAG13250.035.558792
AGATGTA13250.033.9113587
ATTAAGA13150.033.6212233
TTAAGAT13200.032.9417534
ACCGTGC12400.032.7292258
GATCGTC2051.1430529E-831.816918
AGGTCGT1402.7550515E-531.0579366
TCCACCG13100.030.425715
Produced by FastQC (version 0.11.5)