FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_F10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_F10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645135
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA38530.5972393375030033No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG38420.5955342680214218No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT37510.5814286932192486No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA25370.3932510249792679No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT22610.3504692816232261No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG20750.3216381067528502No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC20310.3148178288265247No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA19440.3013322792903811No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA15730.24382493586613654No Hit
GTATAGCCCAGTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGC15370.23824470847187024No Hit
GTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATAGAATGATGGTTA13910.21561378626179017No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT13860.21483875467925317No Hit
CTTATGTACCATGGAGTATGTTTCTGCTCGCTTAAGTAACCTCTACATCC12610.19546296511582847No Hit
CTCTAGAACTTTCTCATCTTGCAAAACTGAAATTCTGTAACTCCCCATGA12250.18988273772156214No Hit
CTAGAAGCCCATCTGTGGCATTCTTAAGATTTCATTTATCTGACAGAGAA12150.18833267455648817No Hit
GTTCTGGTTTTGCATGTAATTTTGTTTCCATGCACATGTCTGTATCAGCA11970.18554256085935503No Hit
CTCATGGACCAAGCAACGGCACTCTGGGGAACAAACAGGACACAAAAACT11420.17701721345144816No Hit
CTCTAGGCACCTTGTGTTAAGTAGAATCACAAAGTATTTCTTTTCGCAAC11420.17701721345144816No Hit
GTTCTAGAGATTGGATACACTCACAAGAGGCAATGTAAGCTTTCCGTAGA11300.17515713765335938No Hit
TTGTACACCTGTGTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGG11050.17128197974067444No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAAGATCAAAT10830.1678718407775117No Hit
CTCTACGGAAAGCTTACATTGCCTCTTGTGAGTGTATCCAATCTCTAGAA10820.1677168344610043No Hit
GTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCATTCTTAAGAT10610.16446170181434894No Hit
GTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTGAGCATCT10400.16120656916769358No Hit
GTACCAACTGAGCCAGCGAGGCACCCATATTTCAACTCTTAAGCGGGAAG10380.16089655653467877No Hit
GTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGGCACACCCCAAGT10300.1596565060026196No Hit
CATAGAGACAGAGAAATAGAATGATGGTTACCAGGGACTAAAGGGAGGTG10230.15857146178706782No Hit
ACTTAGGACTGGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAAT9960.15438629124136807No Hit
TCCGTAGAGTGTCTGAGCCTCTGACAAGAGTTGTAAAACCAGACATGCTC9870.1529912343928015No Hit
CTTAATGACTGAGCCACTCAAGTGCCAACTGTGGCATACCTGTGAGAGCA9700.15035612701217574No Hit
TGTCTGTATCAGCAGAGTTCACAAATACATTGAAAGTGGCTTTTTCTCGG9620.14911607648011657No Hit
GCATTATTTTGACCCATAGTGCTGCTGCTGTTGGATGTATAGCCCAGTCC9390.1455509312004464No Hit
GTCATTAAGTGGCTGCCTTCAGCTCAGGTCATGATCCCAGGGTTCTGGGA9350.14493090593441682No Hit
CCCATATTTCAACTCTTAAGCGGGAAGAAAATGTTGACACCTGCTGTACC9310.14431088066838724No Hit
GTGTAAGTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACC9110.1412107543382393No Hit
CCTTTATTGCCCGGGCTCAGGTCTGGCCCCTGGGTCCTGCTCCCTCTCCT8830.13687057747603215No Hit
GTTCTGGTCTGTTCTGGTTTTGCATGTAATTTTGTTTCCATGCACATGTC8810.13656056484301735No Hit
ATGCTGTACCAACTGAGCCAGCGAGGCACCCATATTTCAACTCTTAAGCG8800.13640555852650996No Hit
GTGTTAAGTAGAATCACAAAGTATTTCTTTTCGCAACTGGCATATCTTAC8750.13563052694397296No Hit
GGTCTGGCCCCTGGGTCCTGCTCCCTCTCCTGCCCTCTAGGCACCTGCCT8190.1269501732195587No Hit
GTTAATATACAGTGCGATATTGGTTTCAGGAGTAGAAATCAGTGATTATT7960.12338502793988854No Hit
ACCTACAACAGTCAAAACACTGCTTACAACTTAGGACTGGGCTATACATC7940.12307501530687374No Hit
GGACTAAAGGGAGGTGGTCATGGGGAGTTACAGAATTTCAGTTTTGCAAG7790.12074992055926279No Hit
ATTTTGACCCATAGTGCTGCTGCTGTTGGATGTATAGCCCAGTCCTAAGT7670.11888984476117401No Hit
AAGTAAGATATGCCAGTTGCGAAAAGAAATACTTTGTGATTCTACTTAAC7610.11795980686212963No Hit
GTCTGTATCAGCAGAGTTCACAAATACATTGAAAGTGGCTTTTTCTCGGT7580.11749478791260744No Hit
TTCTATTTCTCTGTCTCTATGAGCTTGAGGACTCTAGGCACCTTGTGTTA7520.11656475001356305No Hit
GTCTCTATGAGCTTGAGGACTCTAGGCACCTTGTGTTAAGTAGAATCACA7440.11532469948150387No Hit
ATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAAT7380.11439466158245949No Hit
GACTAAAGGGAGGTGGTCATGGGGAGTTACAGAATTTCAGTTTTGCAAGA7320.1134646236834151No Hit
ATCCAACACGGGGCTCCAACTCATGACCCCGAGATCAAGAGCTGTATGCT7170.11113952893580413No Hit
GTATATGGGAGATCACAAACATTACATACAGATGCATGCACACACATGTG7130.11051950366977453No Hit
CCTCTACATCCAACACGGGGCTCCAACTCATGACCCCGAGATCAAGAGCT7050.10927945313771537No Hit
GTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTT7030.10896944050470057No Hit
GCTCTGAGCCTTTTATGTAACAACAGTCAGTGTGCAAATCAGTGTTCTGG7020.10881443418819317No Hit
GTCATGGGGAGTTACAGAATTTCAGTTTTGCAAGATGAGAAAGTTCTAGA6870.1064893394405822No Hit
GAGAAATAGAATGATGGTTACCAGGGACTAAAGGGAGGTGGTCATGGGGA6750.10462926364249343No Hit
CTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCATT6690.10369922574344904No Hit
GCTGTATGCTGTACCAACTGAGCCAGCGAGGCACCCATATTTCAACTCTT6660.10323420679392685No Hit
GAAATAGAATGATGGTTACCAGGGACTAAAGGGAGGTGGTCATGGGGAGT6610.10245917521138985No Hit
ACCAAAAGGTGGGCACACCCCAAGTGTTAACCAACAGATGATAAATCTTA6560.10168414362885289No Hit
AAAGGGAGGTGGTCATGGGGAGTTACAGAATTTCAGTTTTGCAAGATGAG6530.10121912467933068No Hit
GTTCACAAATACATTGAAAGTGGCTTTTTCTCGGTTTATGCTCTCACAGG6520.1010641183628233No Hit
GTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGTTTCTGC6520.1010641183628233No Hit
ATCAAGAGCTGTATGCTGTACCAACTGAGCCAGCGAGGCACCCATATTTC6490.1005990994133011No Hit
GTGTTGGGCACAAGGCATTTGCGGTGCTGCCTTCCTCTCCTTTATTGCCC6470.1002890867802863No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG151.2150883E-4145.4388145
CTGACGG151.2150883E-4145.4388145
TTATACG151.2150883E-4145.4388145
TAGATCG151.2150883E-4145.4388145
CCTCGCG450.0129.27895145
TGCCGTA203.819236E-4109.07911145
GGAGCCG1250.093.08084145
CCGGGCG259.273227E-487.26329145
GGCACCG259.273227E-487.26329145
CTTAACG353.3852222E-583.107895145
AGCGAAA2150.074.2142261
GCGAAAG2300.069.347272
ATGCTCG553.9877905E-666.10855145
GTATATG2650.062.9484371
TGGCGCG350.003571524362.1182679
GTATTGC606.8188474E-660.392768
GGCTCCG501.9799086E-458.175526145
TTTTGCG553.170483E-452.88684145
AAGACGT553.2232035E-452.710495
CAAGACG553.2232035E-452.710494
Produced by FastQC (version 0.11.5)