FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_F08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_F08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066178
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC13850.12990326193187254No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC11730.11001915252425018No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT11430.1072053634571338No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT10840.10167157829180493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2600.066.945331
TAGATCG753.6887468E-758.164017145
CGAAAGC3000.055.5652963
GCGAAAG3050.054.6646462
TGTATCG400.005983768554.528763145
CGGTTCG450.00962665448.3494071
TCTATCG700.001042092541.545723145
GTAGAAA8000.038.057292
TAGTACT5750.035.2912334
ATGAACG2551.8189894E-1234.1049736
AACGTCA3001.8189894E-1128.990599
TGAACGT3001.8189894E-1128.989237
CTAGTAC6550.028.7692853
GAACGTC3052.3646862E-1128.5139968
GTAGCCG2553.0340743E-928.4221469
AGTACTG6850.027.5041565
GACAGTG8200.026.5145367
TCGCAGT1100.00969604226.3501365
AGTAGAA11450.025.969331
GTGTCGG2859.604264E-925.510532145
Produced by FastQC (version 0.11.5)