FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_F07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_F07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055511
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC17250.16342795101140584No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA16040.15196430923031592No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA13910.13178451006195102No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT13570.12856332146230592No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC13490.1278053947329777No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT13390.12685798632131737No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT13000.12316309351584208No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG12970.12287887099234399No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA11890.11264686014641248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2900.057.482071
CGAAAGC3650.049.6326453
GCGAAAG3750.046.376742
GCCGACC951.8821756E-645.925377145
GCGGTAA1401.3780664E-841.55153145
TTATTCG700.00104137141.55153145
CGGTTCG350.00366457841.415651
GTAGAAA8600.038.7669751
TGAACGT2257.9489837E-1032.2106487
ATGAACG2351.2678356E-930.8399836
GAACGTC2401.5879777E-930.1989158
TGGTCGG1000.00598727729.086073145
TAGAAAC11400.027.9684282
TTGCACG1306.3717976E-427.967379145
GGACAGT9650.027.0369196
GCATTAA5450.026.5972081
GACAGTG9700.025.4032447
GTCTAGT4001.8189894E-1225.3670881
TAGTACT7300.024.8210264
GATTAGC4101.8189894E-1224.7483791
Produced by FastQC (version 0.11.5)