FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_F04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_F04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619232
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT169110.6456472737046585No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT103270.3942758793417307No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA91800.3504844168061477No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG71770.27401161867295454No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA69820.2665666882506017No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG63510.2424756569864754No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG53840.2055564379176797No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC51500.19662252141085632No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT50020.1909720101159424No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA49260.18807039620774335No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA49180.18776496316477503No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT46940.17921283796166204No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC45720.17455498405639516No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT45650.1742877301437979No Hit
AAGCAGGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTG43510.1661173962443953No Hit
GTACATGTCTGGCACCTGATAAGAGCTGATAACTGTAGATGGGTTTTGTT42330.1616122588606126No Hit
GCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCAAGGGGTGCTT41970.16023781016725513No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA39240.1498149075759612No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT39100.14928039975076662No Hit
GCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTGATGTC38590.1473332641018436No Hit
CCCTAAATGTAAACCTCCCTCCCTCCCTCCTTTTCCTGCTTTAGAGTGCC37230.14214090237138216No Hit
GCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCTGAGGAAC37070.1415300362854455No Hit
CATCTACAGTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTT36500.1393538258542962No Hit
CCCTTGCACTTTAGAATGCAGGGAAGCGGCTTCTCTCCGTCCACCGCCCC36450.13916293020244103No Hit
CCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGG36220.13828481020390712No Hit
CCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAGCAGAGACAGAGG35200.134390538906061No Hit
CCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGGG33450.127709191091129No Hit
CTTTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAG33440.12767101196075797No Hit
GTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCC33130.12648745891925572No Hit
GTAAAATGGAGACAGTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGAT32540.12423489022736435No Hit
CTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTGATGTCATT32110.12259318762140962No Hit
TTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGAT31910.12182960501398883No Hit
GATAAGAGCTGATAACTGTAGATGGGTTTTGTTTAATTTTTTAAAAGCCT31400.1198824693650658No Hit
GTCCAAATCCCTCATTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGG30620.11690449719612467No Hit
CATAAAGTGCTTTGTACATGTCTGGCACCTGATAAGAGCTGATAACTGTA30190.11526279459016994No Hit
GCCCAAGGCACCATGCTGCTAGGAAAAAGGCTTTTAAAAAATTAAACAAA30160.11514825719905683No Hit
CCTATTGCCCAAGGCACCATGCTGCTAGGAAAAAGGCTTTTAAAAAATTA30060.1147664658953464No Hit
ATGTAAACCTCCCTCCCTCCCTCCTTTTCCTGCTTTAGAGTGCCCTAATG30030.1146519285042333No Hit
GGTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGC29860.11400288328792561No Hit
GGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAACAGGCCCCCAG29760.11362109198421522No Hit
GTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCTGGAAGCCAGAACCAAG29430.1123611806819709No Hit
CCCTCAGCCACCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACAT29330.1119793893782605No Hit
TTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCTGAGGAACAGGTA29310.11190303111751841No Hit
GTCTCAGTCCATTCATCTGTAAAATGGAGACAGTCGTAATACCTGTTCCT29250.11167395633529219No Hit
CGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGGCA28380.10835237199301169No Hit
GTGCAAGGGACTGCAGGGGGTTTGTGGTCCTCAGAGCTTTGCGGGGGAAG28100.10728335634262257No Hit
TATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTG27970.10678702764779906No Hit
GCTCTGAGGACCACAAACCCCCTGCAGTCCCTTGCACTTTAGAATGCAGG27790.10609980330112032No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA27730.10587072851889409No Hit
ACTTACCACTTTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCC27650.10556529547592576No Hit
ACAGTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGT27380.10453445895590768No Hit
TCATTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAA26940.10285457721958192No Hit
CCCTTTACAGGAAATGTGACAAAAATCTTCAGAATTGCTGAAATCGCTGA26930.10281639808921088No Hit
GGTTTACATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAA26760.1021673528729032No Hit
GGACATGCCTTTGCCAGGAGAACTGTTAGCCACTTCCGCCTCCAGTCTCA26670.10182374069956383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCTA900.096.99399145
AGCGAAA5550.061.411241
GTCGCAA651.0925713E-555.782711
GACAGTG53950.052.1166237
GTAGAAA20100.050.148931
GGACAGT58900.048.1067246
CAAGGAC57500.047.393893
AACAAGG61250.045.4640461
ACAAGGA58750.045.2864652
CAGTGGG62550.043.2141349
GCGAAAG8400.040.5628782
CGAAAGC8550.039.841363
AGGACAG74850.038.2413335
ACAGTGG77850.038.1653758
AAGGACA71750.038.078544
TAGAAAC26050.036.4563982
CGCAATA800.002040341536.238683
GGGCTGA36950.036.111571
GAACGTC6850.035.9693538
AATCGCG1051.8180556E-434.640713145
Produced by FastQC (version 0.11.5)