FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_F03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_F03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492411
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACCGAGAGTTCTAGAAGGAACCGCAGTGAGTTCAGGGAGGCCA24390.49531793562694576No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT21900.44475042190365366No Hit
GTCAGAAACTCACTGACCAGGCAGGCAGGCCGATTTTGAGAATCCAAAGT18020.3659544567444675No Hit
AAGCAGGTGTGTGCCATGTTCCCTGCCCTAGCCCCTGAGCTTACTGGTTC16010.3251348974738582No Hit
GTTTAGGACAGTTCATTTTCTTACATATGGTTGTTTACTTGTTCTAATAT15630.3174177668654843No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG15580.31640235494332986No Hit
GTGCATGTGTGCATCACCATGGCTTAGGACCGAGAGTTCTAGAAGGAACC14890.3023896704175983No Hit
TTATAACACTGGCATAAGGATGGACAGAAACAGAGAACAATGAAAAAGAA14510.29467253980922437No Hit
CTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGC14420.2928447983493464No Hit
TATTATAACACTGGCATAAGGATGGACAGAAACAGAGAACAATGAAAAAG14340.2912201392738992No Hit
GGTGAAGAGGGGCAAAGCCAGCCAGTTCTTTGGGCTGATGGGGAAGCGGG14080.28593999727869607No Hit
GTCCTAAACCTCATACCTTCCATAAAAATTACTCAAATGAGATGACAAGA14040.2851276677409725No Hit
TCTTTGGGCTGATGGGGAAGCGGGTGAGAGGAATACCTCCCATCCGGCCG14000.2843153382032489No Hit
GCCCTAGCCCCTGAGCTTACTGGTTCTTGGCTGACTGACAGATTGCTGAA13270.2694903241397938No Hit
GTGCATCACCATGGCTTAGGACCGAGAGTTCTAGAAGGAACCGCAGTGAG12810.26014853445597275No Hit
GCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAGAAGGTGAGCC12330.25040058000328996No Hit
TTGCTGAACCTATCGCACGGGCCCCTGTCTGCACACCTGCTGTCATTCAC12280.24938516808113548No Hit
CAGGAGAACAGGGGCATAGTCAGAAACTCACTGACCAGGCAGGCAGGCCG11580.23516940117097304No Hit
GGTCACTGCAGCTCACAACTGGAACAACGGTGCATGTGTGCATCACCATG11540.23435707163324948No Hit
AAGCAGGCCAGTGGTCAGAAATCAGTTACAGGAGAACAGGGGCATAGTCA10860.2205474694919488No Hit
ATCTATAGAAGGCAGAGAGGATGAGGACCACGGGTCTGAGTGAGCACCCC10810.21953205756979435No Hit
GTGCAGGGCCTTGTGGACAGGGCGCAACTATCTATAGAAGGCAGAGAGGA10800.21932897518536346No Hit
GTTCTAGAAGGAACCGCAGTGAGTTCAGGGAGGCCAGGTGGGACACACCA10420.21161184457698956No Hit
TCCCTGCCCTAGCCCCTGAGCTTACTGGTTCTTGGCTGACTGACAGATTG10320.20958102073268065No Hit
TTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGCCACCTCATTCAGGA10220.20755019688837173No Hit
ATTTATAATAGCTCTAACAAAAGGAGACTTACCACAGGTCACGGACTAAA10130.20572245542849368No Hit
CAACTGGAACAACGGTGCATGTGTGCATCACCATGGCTTAGGACCGAGAG9970.2024731372775994No Hit
CCACCAGAGCATCCGCAAGGACAAATGGTGCAGGGCCTTGTGGACAGGGC9880.2006453958177214No Hit
GTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGC9880.2006453958177214No Hit
GTGGAGGAGAAGGTGAGCCAGCTGAAGGAGGAGGTGCGGCTACAGTATGA9690.19678683051353443No Hit
AAAGCAGGGTCACTGCAGCTCACAACTGGAACAACGGTGCATGTGTGCAT9670.1963806657446726No Hit
GGTGAGAGGAATACCTCCCATCCGGCCGGACAGAAGAACAGCTCCACCAC9520.19333442997820927No Hit
GAACAGGGGCATAGTCAGAAACTCACTGACCAGGCAGGCAGGCCGATTTT9370.1902881942117459No Hit
GGTCAGAAATCAGTTACAGGAGAACAGGGGCATAGTCAGAAACTCACTGA9300.18886661752072964No Hit
GGTCTGGGTCCTGTCCATCAGGATTTTCACAGACTTGGTGTTCTTCATGA9250.18785120559857518No Hit
GGTGTGTGCCATGTTCCCTGCCCTAGCCCCTGAGCTTACTGGTTCTTGGC9220.18724195844528252No Hit
GGCATAAGGATGGACAGAAACAGAGAACAATGAAAAAGAACATAGTCCAG8950.1817587340656484No Hit
CACCAGTGTCAGGCACAGGAGACACACGAGAAGAGAGTTGGTCTGGCCAG8820.1791186630680468No Hit
GCCATGTTCCCTGCCCTAGCCCCTGAGCTTACTGGTTCTTGGCTGACTGA8770.17810325114589234No Hit
GGGTCACTGCAGCTCACAACTGGAACAACGGTGCATGTGTGCATCACCAT8580.17424468584170538No Hit
GGCCAGTGGTCAGAAATCAGTTACAGGAGAACAGGGGCATAGTCAGAAAC8500.17262002676625826No Hit
GTCCTGGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGC8340.169370708615364No Hit
GTCCCAGGACTCTGGAATGATTACTGGAGCTGAAGGCAGACACTTAACTG8320.16896454384650222No Hit
AAGCAGGACATCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCT8180.16612139046446972No Hit
AGCGAAAGCAGGCCAGTGGTCAGAAATCAGTTACAGGAGAACAGGGGCAT8110.16469981377345347No Hit
GAATACCTCCCATCCGGCCGGACAGAAGAACAGCTCCACCACCAGAGCAT8060.163684401851299No Hit
GTATGAGGTTTAGGACAGTTCATTTTCTTACATATGGTTGTTTACTTGTT8020.16287207231357545No Hit
ATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGGAG7930.16104433085369743No Hit
ATAGAAGGCAGAGAGGATGAGGACCACGGGTCTGAGTGAGCACCCCCACT7910.16063816608483564No Hit
GACAAATGGTGCAGGGCCTTGTGGACAGGGCGCAACTATCTATAGAAGGC7870.15982583654711205No Hit
GTCAAGATCTATCTAGTCTGAGTTTCTAAGCCAGGTCTGTGTGTTTATGC7860.15962275416268118No Hit
TTCTTGGCCACCTCATTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTT7800.15840425985609582No Hit
GACTTGGTGTTCTTCATGAAGCTGACGTCTTCTGTCTTGTCGAAGAGCAG7590.1541395297830471No Hit
GTCCAGAAATAGACCCCACAAAATTTGGCAAATTAATTTTTGACAAAGGT7580.1539364473986162No Hit
TAGATAAACTGAGTCTAAAATTCATATTGAAAGGCAAAAGAACTAGGAAA7560.1535302826297544No Hit
GACCAGGCAGGCAGGCCGATTTTGAGAATCCAAAGTGACATAAGATGTGA7540.15312411786089264No Hit
CCTTTGTACAGCAAAAGTTTTTAATTTTAATGAAGTCCCATTTTTAAGTT7520.15271795309203084No Hit
GACATAAGATGTGAAAACTAAATCCAGAAGAAACAGGCATTAAATCGCTT7470.1517025411698764No Hit
CTTGTGGACAGGGCGCAACTATCTATAGAAGGCAGAGAGGATGAGGACCA7470.1517025411698764No Hit
GATGGGGAAGCGGGTGAGAGGAATACCTCCCATCCGGCCGGACAGAAGAA7360.14946863494113657No Hit
TCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGAG7360.14946863494113657No Hit
CTGCAGCTCACAACTGGAACAACGGTGCATGTGTGCATCACCATGGCTTA7350.14926555255670568No Hit
TTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGGGGGAGAAAGGCT7310.14845322301898212No Hit
GAGGAATACCTCCCATCCGGCCGGACAGAAGAACAGCTCCACCACCAGAG7240.14703164632796586No Hit
CAGGTGAAGAGGGGCAAAGCCAGCCAGTTCTTTGGGCTGATGGGGAAGCG7210.1464223991746732No Hit
CAGTTACAGGAGAACAGGGGCATAGTCAGAAACTCACTGACCAGGCAGGC7150.14520390486808785No Hit
GTTCAGGGAGGCCAGGTGGGACACACCAGTGTCAGGCACAGGAGACACAC6930.14073609241060822No Hit
GTTCCCTGCCCTAGCCCCTGAGCTTACTGGTTCTTGGCTGACTGACAGAT6870.13951759810402287No Hit
GTCTGCACACCTGCTGTCATTCACCATCAGCTTCAGCATTTAAACCCTGT6840.13890835095073018No Hit
CTTCTGAGCAGGGAGGCTGTTGCGGGGCTCAGTCCCAGGACTCTGGAATG6840.13890835095073018No Hit
GAAGAACAGCTCCACCACCAGAGCATCCGCAAGGACAAATGGTGCAGGGC6780.13768985664414482No Hit
GGAGAACAGGGGCATAGTCAGAAACTCACTGACCAGGCAGGCAGGCCGAT6700.1360651975686977No Hit
GAAAGCAGGTGTGTGCCATGTTCCCTGCCCTAGCCCCTGAGCTTACTGGT6680.1356590327998359No Hit
CATTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGGGGGAGAAAGG6650.13504978564654324No Hit
GTTTTACATTTAGTCTTGTCATCTCATTTGAGTAATTTTTATGGAAGGTA6640.13484670326211234No Hit
GAGTTCAGGGAGGCCAGGTGGGACACACCAGTGTCAGGCACAGGAGACAC6540.13281587941780343No Hit
GATAGGAGCAGAGGGAGAAAAAGAAGACTCTCTTCTGAGCAGGGAGGCTG6530.13261279703337253No Hit
GCAGGAAGGGCACCGGTGTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGC6440.13078505557349449No Hit
GGCTTAGGACCGAGAGTTCTAGAAGGAACCGCAGTGAGTTCAGGGAGGCC6420.13037889080463272No Hit
TATCTATAGAAGGCAGAGAGGATGAGGACCACGGGTCTGAGTGAGCACCC6330.1285511493447547No Hit
GTGACATAAGATGTGAAAACTAAATCCAGAAGAAACAGGCATTAAATCGC6300.127941902191462No Hit
AGCAGGGTCACTGCAGCTCACAACTGGAACAACGGTGCATGTGTGCATCA6290.1277388198070311No Hit
GATGAGGACCACGGGTCTGAGTGAGCACCCCCACTGCACCCTTCCTGGAG6280.12753573742260024No Hit
GCTCACAACTGGAACAACGGTGCATGTGTGCATCACCATGGCTTAGGACC6270.12733265503816932No Hit
CCTTTATTCTCTTCTAAAAGTTTTACAGTTTTACATTTAGTCTTGTCATC6090.12367717211841328No Hit
GCCTTGTGGACAGGGCGCAACTATCTATAGAAGGCAGAGAGGATGAGGAC5990.12164634827410437No Hit
CCTCCCATCCGGCCGGACAGAAGAACAGCTCCACCACCAGAGCATCCGCA5880.11941244204536454No Hit
GTGCCATGTTCCCTGCCCTAGCCCCTGAGCTTACTGGTTCTTGGCTGACT5820.1181939477387792No Hit
GGCCCCTGTCTGCACACCTGCTGTCATTCACCATCAGCTTCAGCATTTAA5800.1177877829699174No Hit
CCCCTGAGCTTACTGGTTCTTGGCTGACTGACAGATTGCTGAACCTATCG5780.11738161820105562No Hit
ACCCTGAACTGTGTGAACCCCGAGAACGAGAACGCACCTGAGGTGCCAGT5690.11555387674117759No Hit
GTGTCAGGCACAGGAGACACACGAGAAGAGAGTTGGTCTGGCCAGCTGTC5650.11474154720345403No Hit
ATCGAGGACCAGCTCTACAAGCTTGAGTCAGACAAGCGCCTGGTGGAGGA5650.11474154720345403No Hit
CTCACTGACCAGGCAGGCAGGCCGATTTTGAGAATCCAAAGTGACATAAG5620.11413230005016135No Hit
GGGCAAAGCCAGCCAGTTCTTTGGGCTGATGGGGAAGCGGGTGAGAGGAA5600.11372613528129957No Hit
GTTCTTGGCTGACTGACAGATTGCTGAACCTATCGCACGGGCCCCTGTCT5600.11372613528129957No Hit
TTTTAACAGTGTCCTTTGTACAGCAAAAGTTTTTAATTTTAATGAAGTCC5530.11230455859028332No Hit
GTTCAGGGTCAGGGTCTTATAGTCGATCTGCTGCCGGATGAGCTTGTCCT5500.11169531143699066No Hit
CCCTTGTACACGGCATCCAGCAGCTTCTCCTTGACCTGAGTTACCGTGTC5480.11128914666812886No Hit
CACCATGGCTTAGGACCGAGAGTTCTAGAAGGAACCGCAGTGAGTTCAGG5460.11088298189926707No Hit
GATCTATCTAGTCTGAGTTTCTAAGCCAGGTCTGTGTGTTTATGCCAAGT5450.11067989951483619No Hit
GACAAGCGCCTGGTGGAGGAGAAGGTGAGCCAGCTGAAGGAGGAGGTGCG5440.11047681713040529No Hit
GTGGAGGTCCTGGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGC5420.1100706523615435No Hit
GAACAACGGTGCATGTGTGCATCACCATGGCTTAGGACCGAGAGTTCTAG5400.10966448759268173No Hit
GGCATAGTCAGAAACTCACTGACCAGGCAGGCAGGCCGATTTTGAGAATC5390.10946140520825083No Hit
GCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCTCCTTCTTGGCCA5360.10885215805495815No Hit
GTCATTCACCATCAGCTTCAGCATTTAAACCCTGTCAAGATCTATCTAGT5350.10864907567052727No Hit
CTTCCAGATAATCTAACTTGACGTAAACCTTTGAACTTGAAACCATACTT5300.1076336637483728No Hit
GATAAACTGAGTCTAAAATTCATATTGAAAGGCAAAAGAACTAGGAAAAT5270.10702441659508012No Hit
GGGCACCGGTGTGCTGAAGGGGCCTTCTAGCATCTTCCGCAGCTGCTTCT5200.10560283990406387No Hit
GCTTACTGGTTCTTGGCTGACTGACAGATTGCTGAACCTATCGCACGGGC5190.10539975751963299No Hit
CATAAGATGTGAAAACTAAATCCAGAAGAAACAGGCATTAAATCGCTTCC5190.10539975751963299No Hit
GTCCTTTGTACAGCAAAAGTTTTTAATTTTAATGAAGTCCCATTTTTAAG5120.10397818082861675No Hit
GTTCTAATATGATTTGCTGGGAAGACTCTCCTTTCTCTTTTGAATTGTGT5030.1021504393687387No Hit
GTGTTATAATATGAACATTTTCTTGGTTATGTCTTAAATTTAGATACTGT5020.10194735698430782No Hit
GAAGGAGGAGGTGCGGCTACAGTATGAAAAGCTGCACCAGCTGCTGGACG5010.10174427459987694No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACG100.0070251627145.44467145
CGGGTCG151.2147192E-4145.44467145
CATGCGA100.0070251627145.44467145
TTTCGTG202.0973075E-6145.44467145
CGGTTCA100.0070251627145.44467145
TTGTCGG151.2147192E-4145.44467145
TTATACG100.0070251627145.44467145
TAGATCG100.0070251627145.44467145
CTCTGCG151.2147192E-4145.44467145
CGCACCG100.0070251627145.44467145
TTCGACG151.2147192E-4145.44467145
GCCGTAA100.0070251627145.44467145
TGCGTTG100.0070251627145.44467145
GTTAGAC100.0070874146145.016163
AGCGAAA4100.0125.639361
GAGCGAA550.0118.7219541
GCGAAAG5450.094.488862
GCTCCCG406.002392E-790.90292145
CGACGAG259.2704257E-487.26681145
CCGTATG259.2704257E-487.26681145
Produced by FastQC (version 0.11.5)