FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_F02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_F02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046643
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT38770.18943215792886203No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA37570.18356889794653977No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT25160.12293301762935695No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA20480.10006630369830011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC7650.041.6926352
TGACGCG1053.7449117E-641.558014145
GTAGAAA19900.039.7114561
AGCGAAA5900.038.093611
GCGAAAG8550.037.3103181
GGACAGT32800.036.6763046
GAACGTC6050.035.936728
GACAGTG34750.035.24477
TGAACGT6450.034.8308377
CGAACGT1051.8585483E-434.5106629
CAATTCG4100.033.5847639
TAGAAAC25800.030.9058742
TCGCCGG1652.3306384E-630.853678145
CCGACGG1453.4185807E-530.093735145
ATGAACG7700.029.1757666
GTGATCA24650.027.9305329
AGTGATC26000.027.3165158
AGTAGAA21450.026.3639031
GTATAAA5400.025.5095181
ATCGCTG24700.025.027348145
Produced by FastQC (version 0.11.5)