FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833587
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATATATGTATGGATGCTCACGTGGGAGCTCATTATTGATGCTAATT166701.9997912635393786No Hit
ACTTTATGAGGAGCCATGACTGAGGCAACATGAAGAAGGCGATCTCTCAA74000.8877297750564728No Hit
TTCATGCACTCTGCGGGCAGGTATTGAGAATATGCTCTTTGCTCTCTGTG69170.8297874127115705No Hit
GGTATTGAGAATATGCTCTTTGCTCTCTGTGATGTTGTTTTTTCTTCCTC45900.5506323875012445No Hit
ATGTATGGATGCTCACGTGGGAGCTCATTATTGATGCTAATTCATGCACT44190.5301186318884532No Hit
ATTAAACACCATCATCGGTTACAGGAGGGGAGAGAAAGCCATTTATCAGG40750.4888511936966387No Hit
GGTTACAGGAGGGGAGAGAAAGCCATTTATCAGGGCCTCTCGCAGAAGCC39890.4785343341486851No Hit
TTATTGCTTTTTACGTCCTTATTAAACACCATCATCGGTTACAGGAGGGG39660.47577517403702313No Hit
GTTTGTGTGTGATGCCTGAGTGCTCAGTTTATATATGTATGGATGCTCAC39630.47541528358767593No Hit
GATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTCAGAGAAAAAT38990.4677376206682686No Hit
GTGTATATCTCATTTGTGTACTGTTTGTGTGTGATGCCTGAGTGCTCAGT38560.4625791908942918No Hit
ACCCCCAACCACACAACTTTATGAGGAGCCATGACTGAGGCAACATGAAG34660.41579343247915335No Hit
GTATTGAGAATATGCTCTTTGCTCTCTGTGATGTTGTTTTTTCTTCCTCT34480.41363408978307No Hit
CATTTATCTTCTTTTTTTTTCCAGTTAGGTGAAGGAGATGAAAAAAAAAA32860.3942000055183202No Hit
GTAAATAGCAGTGTATATCTCATTTGTGTACTGTTTGTGTGTGATGCCTG32740.39276044372093133No Hit
GAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTCAGAGAAAA32080.38484285383529254No Hit
GAGAAAGAGAGAGAGAATGAATGGCGTCAGAGAAAAATCACGGACGGCGA29730.35665143530309373No Hit
GTATATCTCATTTGTGTACTGTTTGTGTGTGATGCCTGAGTGCTCAGTTT29140.3495735897992651No Hit
TATCAGGGCCTCTCGCAGAAGCCACAACCCCCAACCACACAACTTTATGA28230.3386569128357328No Hit
TTTGTGTACTGTTTGTGTGTGATGCCTGAGTGCTCAGTTTATATATGTAT28060.33661753362276525No Hit
TTATTAAACACCATCATCGGTTACAGGAGGGGAGAGAAAGCCATTTATCA27960.3354178987916078No Hit
CTCAACACATTTATCTTCTTTTTTTTTCCAGTTAGGTGAAGGAGATGAAA27610.331219176882557No Hit
CTGTGATGTTGTTTTTTCTTCCTCTGCCCATCAGGCAGAGAACTGTTTGA27430.3290598341864736No Hit
GTTTAAAGATCAAACAGTTCTCTGCCTGATGGGCAGAGGAAGAAAAAACA27360.3282200898046635No Hit
TATATAAACTGAGCACTCAGGCATCACACACAAACAGTACACAAATGAGA27010.3240213678956126No Hit
CCCTATGTCCTTCTCTCTACCCCTTCTCCCTCTCTCTTTCACTCCTTCTC26170.31394443531389044No Hit
GAATATGCTCTTTGCTCTCTGTGATGTTGTTTTTTCTTCCTCTGCCCATC24690.296189839812761No Hit
GAAAGAATACTGTTTAAAGATCAAACAGTTCTCTGCCTGATGGGCAGAGG21160.2538427302729049No Hit
AGCAGGGAGTGAGTGAAAGAGAGAGAGAGAGAGAGAGAACCCTGGGTTGT20580.24688484825219204No Hit
ATCTCACACTCTCCCTATGTCCTTCTCTCTACCCCTTCTCCCTCTCTCTT19350.23212933982895606No Hit
CATTATTGATGCTAATTCATGCACTCTGCGGGCAGGTATTGAGAATATGC17970.215574379158984No Hit
AAAGCAGGGAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGG17920.2149745617434053No Hit
CTCTACCCCTTCTCCCTCTCTCTTTCACTCCTTCTCTCTCTCCTTCCATT17920.2149745617434053No Hit
CTCTTTGCTCTCTGTGATGTTGTTTTTTCTTCCTCTGCCCATCAGGCAGA17770.21317510949666918No Hit
CATTTGCACGTTATTGCTTTTTACGTCCTTATTAAACACCATCATCGGTT17510.21005605893566No Hit
ATATATGTATGGATGCTCACGTGGGAGCTCATTATTGATGCTAATTCATG17150.2057373735434934No Hit
CCATCATCGGTTACAGGAGGGGAGAGAAAGCCATTTATCAGGGCCTCTCG17130.20549744657726185No Hit
TCTCTCAACACATTTATCTTCTTTTTTTTTCCAGTTAGGTGAAGGAGATG17120.20537748309414613No Hit
ATGCTAATTCATGCACTCTGCGGGCAGGTATTGAGAATATGCTCTTTGCT17010.204057884779873No Hit
TAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTCAGAGAAAAATCA16850.2021384690500212No Hit
GTATGGATGCTCACGTGGGAGCTCATTATTGATGCTAATTCATGCACTCT16640.19961923590459063No Hit
GTTACAGGAGGGGAGAGAAAGCCATTTATCAGGGCCTCTCGCAGAAGCCA16420.19698003927604438No Hit
GAAAGAGAGAGAGAATGAATGGCGTCAGAGAAAAATCACGGACGGCGATC16020.19218149995141479No Hit
GAAGAAGGCGATCTCTCAACACATTTATCTTCTTTTTTTTTCCAGTTAGG15820.1897822302891No Hit
GGTGAAGGAGATGAAAAAAAAAAACAGAAAGAATACTGTTTAAAGATCAA15760.18906244939040556No Hit
GCTTTTTACGTCCTTATTAAACACCATCATCGGTTACAGGAGGGGAGAGA15750.18894248590728982No Hit
ACATGAAGAAGGCGATCTCTCAACACATTTATCTTCTTTTTTTTTCCAGT15690.1882227050085954No Hit
CTGCGGGCAGGTATTGAGAATATGCTCTTTGCTCTCTGTGATGTTGTTTT15660.18786281455924816No Hit
GAATGAATGGCGTCAGAGAAAAATCACGGACGGCGATCATTTGCACGTTA15420.1849836909644704No Hit
GAATAGAATGGAAGGAGAGAGAGAAGGAGTGAAAGAGAGAGGGAGAAGGG15330.18390401961642877No Hit
GACTGAGGCAACATGAAGAAGGCGATCTCTCAACACATTTATCTTCTTTT15010.1800651881567251No Hit
GCCATGACTGAGGCAACATGAAGAAGGCGATCTCTCAACACATTTATCTT14920.17898551680868344No Hit
GACATAGGGAGAGTGTGAGATATGCGTGTCCTGCCAGAGTTGAATAGAAT14310.17166774433862333No Hit
CTCCCTATGTCCTTCTCTCTACCCCTTCTCCCTCTCTCTTTCACTCCTTC14180.1701082190581187No Hit
GTATTCTTTCTGTTTTTTTTTTTCATCTCCTTCACCTAACTGGAAAAAAA14120.16938843815942425No Hit
CTCTTTCACTCCTTCTCTCTCTCCTTCCATTCTATTCCCTCTCTCCCTCT14120.16938843815942425No Hit
GTGTGAGATATGCGTGTCCTGCCAGAGTTGAATAGAATGAAAGAAAGAGA14120.16938843815942425No Hit
GTCCTTCTCTCTACCCCTTCTCCCTCTCTCTTTCACTCCTTCTCTCTCTC13720.1645898988347947No Hit
TCTTTAAACAGTATTCTTTCTGTTTTTTTTTTTCATCTCCTTCACCTAAC13540.16243055613871138No Hit
CCATTTATCAGGGCCTCTCGCAGAAGCCACAACCCCCAACCACACAACTT13430.16111095782443824No Hit
GAATACTGTTTAAAGATCAAACAGTTCTCTGCCTGATGGGCAGAGGAAGA13410.16087103085820675No Hit
TCTCATTTGTGTACTGTTTGTGTGTGATGCCTGAGTGCTCAGTTTATATA13280.15931150557770216No Hit
GGGAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTCAG12800.15355325838814665No Hit
ATATAAAATAGGGTGATATGTGAATTGAATGAAACCCAAAGACTGTATGC12510.1500743173777902No Hit
CTCTGCGGGCAGGTATTGAGAATATGCTCTTTGCTCTCTGTGATGTTGTT12490.14983439041155872No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA12360.1482748651310541No Hit
TCATAAAGTTGTGTGGTTGGGGGTTGTGGCTTCTGCGAGAGGCCCTGATA12200.14635544940120226No Hit
CGTTATTGCTTTTTACGTCCTTATTAAACACCATCATCGGTTACAGGAGG12190.14623548591808652No Hit
TTATTGATGCTAATTCATGCACTCTGCGGGCAGGTATTGAGAATATGCTC12190.14623548591808652No Hit
ACCCTGGGTTGTAAATAGCAGTGTATATCTCATTTGTGTACTGTTTGTGT12140.14563566850250784No Hit
CCACACAACTTTATGAGGAGCCATGACTGAGGCAACATGAAGAAGGCGAT12120.14539574153627635No Hit
GTGAAAGAGAGAGAGAGAGAGAGAGAACCCTGGGTTGTAAATAGCAGTGT12060.14467596063758192No Hit
ATGCCTGAGTGCTCAGTTTATATATGTATGGATGCTCACGTGGGAGCTCA11910.14287650839084584No Hit
TCTCAACACATTTATCTTCTTTTTTTTTCCAGTTAGGTGAAGGAGATGAA11790.14143694659345696No Hit
GTATACTGTTATTGTATTTGCTACAGTTATATGCCATTATGAGCTTGTCG11720.1405972022116468No Hit
TCCTCACACTGGTCATCAGACACAGGCTGCATGGTGGACAAGAGCTTTGA11700.14035727524541528No Hit
AGCCATGACTGAGGCAACATGAAGAAGGCGATCTCTCAACACATTTATCT11540.13843785951556345No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT11530.1383178960324477No Hit
CTTATTAAACACCATCATCGGTTACAGGAGGGGAGAGAAAGCCATTTATC11470.13759811513375328No Hit
ATTTTATATGGTCCTTCGAGCCGGATATTACATTTACCGCAGTTAAAGAT11260.13507888198832277No Hit
AATTCATGCACTCTGCGGGCAGGTATTGAGAATATGCTCTTTGCTCTCTG11180.13411917412339683No Hit
ATTCTATTCCCTCTCTCCCTCTTTCTATAGCTACCCTCTCCCTCTCTCTC10950.13136001401173483No Hit
CAGGAGGGGAGAGAAAGCCATTTATCAGGGCCTCTCGCAGAAGCCACAAC10940.1312400505286191No Hit
GTTCTCTGCCTGATGGGCAGAGGAAGAAAAAACAACATCACAGAGAGCAA10900.13076019659615612No Hit
GCTCATTATTGATGCTAATTCATGCACTCTGCGGGCAGGTATTGAGAATA10890.13064023311304038No Hit
CATCAGAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTT10860.13028034266369318No Hit
GAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTCAGAG10840.1300404156974617No Hit
GTTGTAAATAGCAGTGTATATCTCATTTGTGTACTGTTTGTGTGTGATGC10830.12992045221434595No Hit
CCTTCACCTAACTGGAAAAAAAAAGAAGATAAATGTGTTGAGAGATCGCC10690.1282409634507256No Hit
GAGAGAGAGAATGAATGGCGTCAGAGAAAAATCACGGACGGCGATCATTT10670.12800103648449412No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC10570.12680140165333673No Hit
CTTTATGAGGAGCCATGACTGAGGCAACATGAAGAAGGCGATCTCTCAAC10450.12536183985594784No Hit
AGCAGGGAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCG10170.12200286232870715No Hit
TCCTTCACCTAACTGGAAAAAAAAAGAAGATAAATGTGTTGAGAGATCGC10130.12152300839624419No Hit
CGTGGGAGCTCATTATTGATGCTAATTCATGCACTCTGCGGGCAGGTATT10130.12152300839624419No Hit
GAATGGAAGGAGAGAGAGAAGGAGTGAAAGAGAGAGGGAGAAGGGGTAGA10020.12020341008197105No Hit
TTCTTCCTCTGCCCATCAGGCAGAGAACTGTTTGATCTTTAAACAGTATT9820.11780414041965626No Hit
GAGCCATGACTGAGGCAACATGAAGAAGGCGATCTCTCAACACATTTATC9820.11780414041965626No Hit
GCCCATCAGGCAGAGAACTGTTTGATCTTTAAACAGTATTCTTTCTGTTT9790.11744424997030904No Hit
AAATAGCAGTGTATATCTCATTTGTGTACTGTTTGTGTGTGATGCCTGAG9730.1167244690716146No Hit
CAGGGAGAGAGATAATGAGAGAGAGAAAGAGAGAGAGAATGAATGGCGTC9660.11588472468980443No Hit
TTCTATAGCTACCCTCTCCCTCTCTCTCTCTCTCCTCTCACGCTCTCTCT9620.11540487075734147No Hit
GGAGGGGAGAGAAAGCCATTTATCAGGGCCTCTCGCAGAAGCCACAACCC9410.11288563761191094No Hit
AGCCACAACCCCCAACCACACAACTTTATGAGGAGCCATGACTGAGGCAA9400.11276567412879519No Hit
CCCCCAACCACACAACTTTATGAGGAGCCATGACTGAGGCAACATGAAGA9400.11276567412879519No Hit
CATAGGGAGAGTGTGAGATATGCGTGTCCTGCCAGAGTTGAATAGAATGA9380.11252574716256372No Hit
GGCAGAGAACTGTTTGATCTTTAAACAGTATTCTTTCTGTTTTTTTTTTT9340.11204589323010077No Hit
GGACATAGGGAGAGTGTGAGATATGCGTGTCCTGCCAGAGTTGAATAGAA9330.11192592974698502No Hit
TCCATACATATATAAACTGAGCACTCAGGCATCACACACAAACAGTACAC9310.11168600278075355No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA9250.1109662218820591No Hit
CTGTTTGTGTGTGATGCCTGAGTGCTCAGTTTATATATGTATGGATGCTC9180.11012647750024893No Hit
GTGCTCAGTTTATATATGTATGGATGCTCACGTGGGAGCTCATTATTGAT9140.10964662356778597No Hit
AGATATGCGTGTCCTGCCAGAGTTGAATAGAATGAAAGAAAGAGAGAGAG9130.10952666008467021No Hit
TGTGTACTGTTTGTGTGTGATGCCTGAGTGCTCAGTTTATATATGTATGG9070.10880687918597579No Hit
GAGTAGCTATAGCAAGAGGGAGAGAGGGAATAGAATGGAAGGAGAGAGAG9020.1082070617703971No Hit
TTTTACGTCCTTATTAAACACCATCATCGGTTACAGGAGGGGAGAGAAAG8950.1073673173885869No Hit
AAAGCAGGGAGTGAGTGAAAGAGAGAGAGAGAGAGAGAGAACCCTGGGTT8950.1073673173885869No Hit
GAGAGAGAGAGAACCCTGGGTTGTAAATAGCAGTGTATATCTCATTTGTG8880.10652757300677673No Hit
GGATGTGAATGTCCTGTCAGCCAGTGGCGAGATAGGGAGAGAGGGAATAG8850.10616768255742952No Hit
GAGTGAAAGAGAGAGAGAGAGAGAGAGAACCCTGGGTTGTAAATAGCAGT8690.10424826682757769No Hit
CTCTGTGATGTTGTTTTTTCTTCCTCTGCCCATCAGGCAGAGAACTGTTT8680.10412830334446195No Hit
GAGGGAATAGAATGGAAGGAGAGAGAGAAGGAGTGAAAGAGAGAGGGAGA8660.10388837637823048No Hit
CTCTCTTTCTTTCATTCTATTCAACTCTGGCAGGACACGCATATCTCACA8570.10280870503018881No Hit
TATTAAACACCATCATCGGTTACAGGAGGGGAGAGAAAGCCATTTATCAG8570.10280870503018881No Hit
CGCATATCTCACACTCTCCCTATGTCCTTCTCTCTACCCCTTCTCCCTCT8490.10184899716526291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAG1950.0111.89963145
AGCGAAA5550.0109.7149661
TTCTTCG553.6379788E-12105.79602145
AGTATTA2450.0103.90681145
GCGAAAG6350.097.0108952
TTGCTCG405.999955E-790.91845145
CGAAAGC7350.083.802073
GCTGCCA406.556662E-572.734764145
ACGATAG809.640644E-1172.734764145
GTTAGGA300.001910960772.73476145
CTTCTGG300.001910960772.73476145
CCTCAAC300.001939430772.464143
GCCCATA955.456968E-1268.90662145
AGGCCCG651.366825E-767.13978145
GGTGTAA1250.063.791421
GAAAGCA9950.062.6323244
ACTGTCG350.003520907862.34408145
CGATACG702.2824497E-762.34408145
GGTGCCC1400.062.1121258
ATGGGCG954.4383341E-1061.250324145
Produced by FastQC (version 0.11.5)