FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_E10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_E10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228892
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG350.003520075762.349586145
AGCGAAA5900.052.8183481
TCGTGCG1850.047.0048339
CGAAAGC7000.046.5794563
GACCGTC907.5060925E-540.257137
GCGAAAG8350.037.3192252
TACTCCG1251.2368584E-534.915764145
TATACGC2750.034.257952
GTATATA7500.033.8201181
CTATACG2850.033.0572621
ATACGCA2850.033.0505333
TACGGCA1000.00609386328.9815924
AGCGTAG1804.721398E-628.1788436
TATATAC9650.027.7814143
CCGGATA3153.274181E-1127.710928145
GTATATG1958.783205E-626.0154741
AAGCGTA2051.2969425E-524.7403835
TATACAG5100.024.1513295
TAGTACT2401.6074355E-624.1513274
TACGCAC3952.3646862E-1123.8456154
Produced by FastQC (version 0.11.5)