FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347057
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGAGATGGTTGATAATAGGACTTCATGACTCTATGTCTGCAAAG23750.17631028234143023No Hit
GGGTAAGGGTGTGAGGCCTGGGCTCTGGGCCAATTCTATCACCACTTAGT23250.17259848692371593No Hit
ATCTATATGTGGATAGTCTTTTTTTAAGTCGGCTCCATGCCCAGCGTGGA23250.17259848692371593No Hit
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC19660.1459477958245271No Hit
GTGATGGAGGGACCCAGACAAGATGACCGGAGCCCCACCCAGCCTTGCTG19190.14245870813187564No Hit
CCTCTATGATGCGCAGCAGGGCCTTGACGCCGGGTCTCCACAGGTGGCGC16770.12449361831013833No Hit
GTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAA15860.11773815064989826No Hit
TTATTATTGGGCTCAGGTGATGGCTGTGTGAAGATTCACTACTCCATTCC15610.1158822529410411No Hit
AACCTGAACCTACCTATTTTTTTTTAAGTGCTTCAAAGAAATGTCTCCCT14490.10756783120536102No Hit
TACCAAGACTTACTTGATTGGAATACCATTTTAAAAACACTCTGGGTAGG14090.10459839487118956No Hit
CATATGGGCCACAAATTTCAAGTTGTATAGCTTGAATAAGGGAAGCAATT13620.10110930717853811No Hit
TAATAGGACTTCATGACTCTATGTCTGCAAAGAACATGCACCATGAATAC13580.10081236354512095No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGG100.007028605145.43019145
AGCGAAA6350.0109.641491
TTGTGCG203.8210535E-4109.07264145
CTTGGCG259.2776277E-487.25812145
ATACACG353.3875644E-583.10297145
GGTAAGG3550.075.5679862
GAATGGT300.001913269972.715096145
AGTAGCG300.001913269972.715096145
ATGTTCG300.001913269972.715096145
GTGCGTA300.001933424672.523281
TGATTCG350.003525155662.327225145
CCGACGG350.003525155662.327225145
TGAATCG350.003525155662.327225145
GAGGGCG400.00598083254.53632145
CGAAAGC13050.054.4336133
GCTACCG553.2267053E-452.7030839
CTGGCCG701.6668217E-551.939354145
ACTGACG450.0095276948.47673145
TCGGTGT450.0095276948.47673145
TAAGGGT6500.045.7114834
Produced by FastQC (version 0.11.5)