FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_E08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_E08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196639
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT14580.12184125705413246No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG300.001911672872.7299145
CGATACG406.56001E-572.7299145
TGACGCG553.9864644E-666.11809145
TAGACCG451.1965267E-464.416415
GTCGTAC350.00356396262.154791
CGGATTG400.00597585554.54742145
CGATACA1056.7380824E-848.486603145
AGCGAAA3250.044.623961
CCGACGG1053.7424052E-641.559944145
ACGATAG700.001040390941.55994145
CAATTCG800.002005365436.36495145
GCGAAAG4550.035.061681
CGAAAGC4850.032.8846632
GTCTAGT3700.029.3975371
GCTATTG7950.029.1709168
CTATTGA8100.028.6307149
ACCGTGC5600.028.4709438
GTAGAAA11050.027.5618531
TGTCTCG1906.983071E-626.795225145
GCATTAA4900.026.637771
Produced by FastQC (version 0.11.5)