FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_E07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_E07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905517
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT23180.12164677617675414No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC20710.1086844147808705No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT20010.10501087106543788No Hit
CCCTACACCTGCTTCAACAGGAGCGTGAAGAAAAGACACACACCAAACTG19200.100760056194723No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG350.003524026662.333267145
AGCGAAA4150.054.1584971
GCGAAAG4850.047.8178672
CGAAAGC5450.042.54573
TAGATCG1609.822543E-1040.906208145
TAGACCG800.002041461236.2341235
TGTCTCG1854.0636223E-935.378338145
TGACGCG1352.0988406E-532.320953145
GTAGAAA15000.031.9011041
ATGAACG2859.094947E-1230.514556
CACATCG3500.029.088856145
GACGATG2903.8198777E-1027.584263145
ACCGTGC8500.027.2850288
TACCGTG1358.1011525E-426.8429137
GCGCGCG1100.00953054826.444414145
GTCTAGT4950.026.3645481
GTTATCG1400.001001736625.8842379
CAAGACG5650.025.742352145
GTATAGG7050.024.6817051
ATAGGAG16050.023.9278133
Produced by FastQC (version 0.11.5)