FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_E06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_E06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786480
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAATGGCTGAGCCAAGCCTTGAATGGCATGTCAGGTAGGCCAGGAAA19690.11021673906229008No Hit
GTTCAAAGGCGCGCAAAGTAGAGCAGTACTGTGTTCCCATTCGGTACCCC19290.10797769916259907No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG19130.10708208320272267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG404.7184585E-9109.07634145
TGACGCG651.3699719E-767.12391145
AGCGAAA4750.054.9532471
AACGACG553.1724077E-452.885494145
CGAAAGC5350.048.757493
AACGCGC450.00965763748.311446
GCGAAAG6100.042.7734952
CGGTTCG1458.6962245E-735.0037461
GTAGAAA15400.032.4872441
AGTAGAA15100.030.7317661
ATGAACG2451.9845174E-929.5784346
GATTAGC5050.028.7159441
CACATCG3005.675247E-1026.663105145
GTATACC3108.6583896E-1025.7112734
CTATACC2559.546966E-825.5737824
TAGGGTG4400.024.7033315
TATAGTA7650.024.6328092
ACCGTGC8250.024.5962928
TAGATCG1500.001468873924.239187145
CGACCAA3052.0480002E-823.7583945
Produced by FastQC (version 0.11.5)