FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_E02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_E02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037312
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT53540.5161417201382034No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT35810.34521918188548867No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA24050.2318492411154985No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA19390.18692543805528133No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG18600.17930960019743336No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG18550.1788275851431392No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT18140.17487506169792694No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG18070.1742002406219151No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA16350.15761892275419548No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA15350.14797862166831194No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA14350.13833832058242843No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC13550.13062607971372162No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA13460.12975845261599211No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT13420.12937284057255677No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT12830.12368506293188548No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT12830.12368506293188548No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC12720.12262462981243831No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC11820.11394835883514313No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA11710.11288792571569595No Hit
GCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCAAGGGGTGCTT11500.11086346248766041No Hit
GTACATGTCTGGCACCTGATAAGAGCTGATAACTGTAGATGGGTTTTGTT11480.11067065646594273No Hit
CCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGGG11450.11038144743336625No Hit
GCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCTGAGGAAC11270.10864619323790721No Hit
GCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTGATGTC11240.1083569842053307No Hit
CCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGG11240.1083569842053307No Hit
CCCTTGCACTTTAGAATGCAGGGAAGCGGCTTCTCTCCGTCCACCGCCCC11130.10729655108588353No Hit
AAGCAGGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTG11040.106428923988154No Hit
CATCTACAGTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTT10730.10344043065153011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG100.0070273555145.43723145
ACGATAG450.00952525648.479073145
GTCGTAG450.00952525648.479073145
GTAGAAA8800.046.9405631
AGCGAAA3550.042.869371
GACAGTG18850.042.6703157
GCGAAAG3900.040.8743972
GGACAGT19850.040.5187266
CAAGGAC19550.038.918563
GACGATG1001.367055E-436.359306145
CGAAGTA1604.614776E-836.2278375
CGAAAGC4450.035.8242383
AGTAGAA6600.035.13681
ATGAACG3150.034.5043646
ACCATCG4550.033.44438
CCATCGG4550.033.44439
ACAAGGA22800.033.0515482
TAGAAAC12650.032.6495862
AACAAGG24650.032.633391
CGATACA900.003575184632.31938145
Produced by FastQC (version 0.11.5)