FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_E01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_E01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311496
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG39550.30156401544495753No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA36730.2800618530289074No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT34940.2664133173109182No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT31390.23934499228362113No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC31150.23751502101417007No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA27080.2064817582363957No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA26550.20244057168302457No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG24280.1851320934261332No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC23910.18231088771906281No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT23580.1797946772235676No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG23310.17773595954543514No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT23200.1768972227136034No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG22850.17422851461232058No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG22690.17300853376601988No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT21310.1624861989666762No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA20810.1586737588219865No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT20320.15493756748019055No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA20240.1543275770570402No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT19610.14952390247473113No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA19200.14639770155608559No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT17840.1360278643625295No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG17620.134350390698866No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA17410.13274916583809634No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA17300.1319104290062646No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT17190.13107169217443287No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG17180.13099544337153907No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC16920.12901297449630042No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT16890.12878422808761902No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG16830.12832673527025626No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT16570.12634426639501758No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG15660.11940562533168231No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA15290.11658441962461188No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA15200.11589818039856774No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA15060.11483069715805462No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG14680.11193324264809042No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT14330.10926453454680762No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA14210.10834954891208208No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT13980.10659582644552479No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA13610.10377462073845439No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA13500.10293588390662266No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC13420.1023258934834723No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT13370.10194464946900333No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA13360.10186840066610954No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT13180.10049592221402125No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT13160.10034342460823364No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT13140.10019092700244606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG202.1005726E-6145.41759145
TGACGCG256.3738444E-6116.33407145
ACTAACG259.280765E-487.25055145
AGTAGAA5050.077.534451
TATCGCG300.001913916272.7088145
TGGACCG902.746674E-1064.63004145
AGCGAAA1250.063.807981
GTTGACG350.00352634562.321827145
GTACCCG350.00352634562.321827145
TCGTATG350.00352634562.321827145
GTAGAAA6500.059.1114962
CCTTATA9400.050.1296842
AGATACG450.00953089648.47253145
ATCGTTA450.00953089648.47253145
GTTGGCG450.00953089648.47253145
AAAGACG1402.6557245E-1046.74137145
CAATTCG2200.046.126299
GCGAAAG1751.8189894E-1245.568432
CGAAAGC1751.8189894E-1245.5632173
TCTCGAG803.6723835E-545.442997145
Produced by FastQC (version 0.11.5)