FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_D10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_D10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219976
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG855.241322E-542.78505145
AGCGAAA4100.038.908261
CTACGAT900.0036368232.207234
GTGTACG2051.1374141E-831.8340321
GCGAAAG5700.027.9786112
TATACAG7000.027.9489845
CCTTATA6300.027.6152522
CGAAAGC6000.026.5731433
TATATAC11600.023.7408983
GTATATA11500.023.3295761
TATACTG8950.022.6691515
TTATACT7400.022.52334
CTAATAC5051.8189894E-1221.5263983
CAGCTCG7100.021.4345639
TAGACAG7950.020.0519145
CTATTAT6150.020.0436481
TGTACGC2307.9706725E-418.9104442
TAATAGT5801.0913936E-1118.7412784
CTAGTAC2359.225384E-418.5035423
GTATAAG5209.131327E-1018.1277121
Produced by FastQC (version 0.11.5)