FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R1_D09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R1_D09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311955
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT16900.1288153938206722No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA15670.11944007225857595No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA15670.11944007225857595No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG14790.11273252512471844No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC13890.10587253373781876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG300.001937786472.481995
CGAAAGC2750.071.166853
AGCGAAA3650.053.6577221
CAATTCG3850.052.7141769
GGTAACC450.00965072348.319486
TTATACG657.2285254E-444.75102145
TAGGCTA850.002745637734.109175
GCGAAAG5750.034.0415082
CCTTATA4700.033.9342082
GTATAGG1751.0050462E-733.1599041
GGGTATA1102.429216E-432.9714971
AAGTGCG1102.4401184E-432.9463589
TATAGGG2101.4413672E-831.0696322
TAGACTA1402.7519905E-531.063715
AGGTCGT2901.2732926E-1129.9914026
GTAGAAA8200.028.2910462
TTATACT6150.028.2888894
TGTAATA4650.028.0628932
GCACCGT2654.5747583E-927.350656
GTGTAGG2101.549365E-524.1790981
Produced by FastQC (version 0.11.5)